In:
Journal of Cellular Physiology, Wiley, Vol. 226, No. 9 ( 2011-09), p. 2207-2212
Abstract:
We previously identified a poptosis‐ i nduced r egulator ( air ) as a pro‐apoptotic transcript whose expression was repressed by NF‐κB/Rel activity in the human leukemia cell line Jurkat (Turco, Lamberti, Bisogni, Romano, Petrella, Ammirante, Rosati, d'Avenia, Arra, Spugnini, Venuta, 2007, Leukemia 21:2557–2559). In this paper, we report that air sequence is detectable by Southern blot in human healthy donors (HHD) leukocytes and in two pancreatic adenocarcinoma cell lines (AsPC‐1 and PANC‐1), providing the first definitive evidence of air gene presence in human genome. In addition, we demonstrate that air expression is induced in the tumor cell lines by a naturally occurring ROS‐inducing compound, phenethyl isothiocyanate (PEITC), a potential dietary cancer chemopreventive agent. Since PEITC inhibits NF‐κB activation, air induction by this agent is consistent with the suppressive effect of NF‐κB on air expression. This finding contributes a new clue to the role of NF‐κB in regulating oxidative stress‐induced pro‐apoptotic genes and identifies a novel potential tool for enhancing pancreas cancer response to treatment. J. Cell. Physiol. 226: 2207–2212, 2011. © 2011 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0021-9541
,
1097-4652
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
1478143-8
SSG:
12
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