In:
European Journal of Biochemistry, Wiley, Vol. 212, No. 3 ( 1993-03), p. 785-789
Abstract:
Many proteins have a blocked α‐amino group which renders them inaccessible to sequence analysis by the classical Edman degradation procedure. Blockage typically occurs when the α‐amino groups are acylated with acetyl or formyl groups or when the N‐terminal residue is pyrrolidone carboxylic acid formed by cyclization of glutamine. We have found that N ‐formyl groups of proteins and peptides can be removed by exposure to hydrazine vapor at‐5°C for 8 h. Under these conditions, peptide‐bond cleavage or modification of the constituent amino‐acid residues does not occur. Deblocking of N‐terminal pyrrolidone carboxylate residues by conversion to γ‐hydrazidyl glutamic acid can be achieved by exposure to hydrazine vapor at 20°C for 4 h. These conditions cause partial modification of asparagine and glutamine residues to their corresponding hydrazides, and conversion of arginine residues to ornithine.
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1993.212.issue-3
DOI:
10.1111/j.1432-1033.1993.tb17719.x
Language:
English
Publisher:
Wiley
Publication Date:
1993
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
2172518-4
SSG:
12
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