In:
American Journal of Physiology-Cell Physiology, American Physiological Society, Vol. 288, No. 5 ( 2005-05), p. C1030-C1041
Abstract:
The inhibitory control of pancreatic ductal HCO 3 − secretion may be physiologically important in terms of limiting the hydrostatic pressure developed within the ducts and in terms of switching off pancreatic secretion after a meal. Substance P (SP) inhibits secretin-stimulated HCO 3 − secretion by modulating a Cl − -dependent HCO 3 − efflux step at the apical membrane of the duct cell (Hegyi P, Gray MA, and Argent BE. Am J Physiol Cell Physiol 285: C268–C276, 2003). In the present study, we have shown that SP is present in periductal nerves within the guinea pig pancreas, that PKC mediates the effect of SP, and that SP inhibits an anion exchanger on the luminal membrane of the duct cell. Secretin (10 nM) stimulated HCO 3 − secretion by sealed, nonperfused, ducts about threefold, and this effect was totally inhibited by SP (20 nM). Phorbol 12,13-dibutyrate (PDBu; 100 nM), an activator of PKC, reduced basal HCO 3 − secretion by ∼40% and totally blocked secretin-stimulated secretion. In addition, bisindolylmaleimide I (1 nM to 1 μM), an inhibitor of PKC, relieved the inhibitory effect of SP on secretin-stimulated HCO 3 − secretion and also reversed the inhibitory effect of PDBu. Western blot analysis revealed that guinea pig pancreatic ducts express the α-, β I -, δ-, ε-, η-, θ-, ζ-, and μ-isoforms of PKC. In microperfused ducts, luminal H 2 DIDS (0.5 mM) caused intracellular pH to alkalinize and, like SP, inhibited basal and secretin-stimulated HCO 3 − secretion. SP did not inhibit secretion further when H 2 DIDS was present in the lumen, suggesting that SP and H 2 DIDS both inhibit the activity of an anion exchanger on the luminal membrane of the duct cell.
Type of Medium:
Online Resource
ISSN:
0363-6143
,
1522-1563
DOI:
10.1152/ajpcell.00430.2003
Language:
English
Publisher:
American Physiological Society
Publication Date:
2005
detail.hit.zdb_id:
1477334-X
SSG:
12
Bookmarklink