In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2019-04-11)
Abstract:
Symbiosis is a major force of evolutionary change, influencing virtually all aspects of biology, from population ecology and evolution to genomics and molecular/biochemical mechanisms of development and reproduction. A remarkable example is Wolbachia endobacteria, present in some parasitic nematodes and many arthropod species. Acquisition of genomic data from diverse Wolbachia clades will aid in the elucidation of the different symbiotic mechanisms(s). However, challenges of de novo assembly of Wolbachia genomes include the presence in the sample of host DNA: nematode/vertebrate or insect. We designed biotinylated probes to capture large fragments of Wolbachia DNA for sequencing using PacBio technology (LEFT-SEQ: Large Enriched Fragment Targeted Sequencing). LEFT-SEQ was used to capture and sequence four Wolbachia genomes: the filarial nematode Brugia malayi , w Bm, (21-fold enrichment), Drosophila mauritiana flies (2 isolates), w Mau (11-fold enrichment), and Aedes albopictus mosquitoes, w AlbB (200-fold enrichment). LEFT-SEQ resulted in complete genomes for w Bm and for w Mau. For w Bm, 18 single-nucleotide polymorphisms (SNPs), relative to the w Bm reference, were identified and confirmed by PCR. A limit of LEFT-SEQ is illustrated by the w AlbB genome, characterized by a very high level of insertion sequences elements (ISs) and DNA repeats, for which only a 20-contig draft assembly was achieved.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-019-42454-w
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2019
detail.hit.zdb_id:
2615211-3
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