In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 6 ( 2022-6-10), p. e0009900-
Abstract:
Bejel (endemic syphilis) is a neglected non-venereal disease caused by Treponema pallidum subsp. endemicum (TEN). Although it is mostly present in hot, dry climates, a few cases have been found outside of these areas. The aim of this work was the sequencing and analysis of TEN isolates obtained from “syphilis patients” in Cuba, which is not considered an endemic area for bejel. Genomes were obtained by pool segment genome sequencing or direct sequencing methods, and the bioinformatics analysis was performed according to an established pipeline. We obtained four genomes with 100%, 81.7%, 52.6%, and 21.1% breadth of coverage, respectively. The sequenced genomes revealed a non-clonal character, with nucleotide variability ranging between 0.2–10.3 nucleotide substitutions per 100 kbp among the TEN isolates. Nucleotide changes affected 27 genes, and the analysis of the completely sequenced genome also showed a recombination event between tprC and tprI , in TP0488 as well as in the intergenic region between TP0127–TP0129. Despite limitations in the quality of samples affecting breadth of sequencing coverage, the determined non-clonal character of the isolates suggests a persistent infection in the Cuban population rather than a single outbreak caused by imported case.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0009900
DOI:
10.1371/journal.pntd.0009900.g001
DOI:
10.1371/journal.pntd.0009900.g002
DOI:
10.1371/journal.pntd.0009900.g003
DOI:
10.1371/journal.pntd.0009900.g004
DOI:
10.1371/journal.pntd.0009900.t001
DOI:
10.1371/journal.pntd.0009900.t002
DOI:
10.1371/journal.pntd.0009900.t003
DOI:
10.1371/journal.pntd.0009900.s001
DOI:
10.1371/journal.pntd.0009900.s002
DOI:
10.1371/journal.pntd.0009900.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5
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