In:
Journal of Medical Microbiology, Microbiology Society, Vol. 57, No. 12 ( 2008-12-01), p. 1466-1472
Kurzfassung:
The Candida antigen CR3-RP (complement receptor 3-related protein) is supposed to be a ‘mimicry’ protein because of its ability to bind antibody directed against the α subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immunization with CR3-RP in vivo in a rabbit animal model, and (ii) determine the role of CR3-RP in the adherence of Candida albicans in vitro using the model systems of buccal epithelial cells (BECs) and biofilm formation. The synthetic C. albicans peptide DINGGGATLPQ corresponding to 11 amino-acids of the CR3-RP sequence DINGGGATLPQALXQITGVIT, determined by N-terminal sequencing, was used for immunization of rabbits to obtain polyclonal anti-CR3-PR serum and for subsequent characterization of the humoral isotypic response of rabbits. A significant increase of IgG, IgA and IgM anti-CR3-RP specific antibodies was observed after the third ( P 〈 0.01) and the fourth ( P 〈 0.001) immunization doses. The elevation of IgA levels suggested peptide immunomodulation of the IgA1 subclass, presumably in coincidence with Candida epithelial adherence. Blocking CR3-RP with polyclonal anti-CR3-RP serum reduced the ability of Candida to adhere to BECs, in comparison with the control, by up to 35 % ( P 〈 0.001), and reduced biofilm formation by 28 % ( P 〈 0.001), including changes in biofilm thickness and integrity detected by confocal laser scanning microscopy. These properties of CR3-RP suggest that it has potential for future vaccine development.
Materialart:
Online-Ressource
ISSN:
0022-2615
,
1473-5644
DOI:
10.1099/jmm.0.2008/001479-0
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2008
ZDB Id:
2083944-3
SSG:
12
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