In:
Skin Pharmacology and Physiology, S. Karger AG, Vol. 4, No. 3 ( 1991), p. 165-168
Abstract:
Single-cell suspensions of murine and human epidermal cells were studied for the presence of peptidoleukotrienes (LTs), using in vitro guinea pig ileum contraction as bioassay and a commercial radioimmunoassay. The calcium ionophore A23187 at 5 & #215; 10 〈 sup 〉 -6 〈 /sup 〉 〈 i 〉 M 〈 /i 〉 alone or in combination with arachidonic acid at 10 〈 sup 〉 -4 〈 /sup 〉 〈 i 〉 M 〈 /i 〉 caused release of LTC 〈 sub 〉 4 〈 /sub 〉 /D 〈 sub 〉 4 〈 /sub 〉 within 10–30 min and for up to 18 h. LTB 〈 sub 〉 4 〈 /sub 〉 , as was measured in the chemotaxis assay, was released at different levels and with different kinetics from the same cells. Epidermal cells may thus regulate cutaneous inflammation by secreting potent vasoactive and muscle-contracting in addition to chemotactic lipid mediators.
Type of Medium:
Online Resource
ISSN:
1660-5527
,
1660-5535
Language:
English
Publisher:
S. Karger AG
Publication Date:
1991
detail.hit.zdb_id:
1483572-1
SSG:
15,3
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