In:
Journal of the chemical society of pakistan, Chemical Society of Pakistan, Vol. 43, No. 1 ( 2021), p. 80-80
Abstract:
To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.
Type of Medium:
Online Resource
ISSN:
0253-5106
Uniform Title:
Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method
DOI:
10.52568/000551/JCSP/43.01.2021
Language:
Unknown
Publisher:
Chemical Society of Pakistan
Publication Date:
2021
detail.hit.zdb_id:
3024078-5
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