In:
Frontiers in Veterinary Science, Frontiers Media SA, Vol. 9 ( 2023-1-5)
Abstract:
In animal husbandry, feed efficiency is a crucial economic trait. In this study, the general linear model was used to perform association analysis for various genotypes and feed conversion ratio (FCR)-related traits. Reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression of SHISA3 and RFC3 mRNA levels in 10 tissues from 6 sheep. The results showed that SNPs in the NC_040257.1:c.625 T & gt; C and NC_040261.1:g.9905 T & gt; C were analyzed whether they were associated to feed efficiency parameters in Hu sheep (body weight, feed intake, average daily growth, and feed conversion ratio). NC_040257.1:c.625 T & gt; C was shown to be significantly associated with body weight at 80, 100, and 120 days as well as feed conversion ratio ( P & lt; 0.05), whereas NC_040261.1:g.9905 T & gt; C was found to be significantly associated with average daily weight gain from 80-140 days (ADG80-140) and FCR ( P & lt; 0.05). In Hu sheep, the CC genotypes of SHISA3 and RFC3 were the most common genotypes related to feed efficiency traits. Furthermore, the feed conversion ratio of the combined genotypes TT SHISA 3 -CC RFC 3 , TT SHISA 3 -CT RFC 3 , TT SHISA 3 -TT RFC 3 , CT SHISA 3 -CC RFC 3 and CT SHISA 3 -CT RFC 3 was significantly better than the FCR of CC SHISA 3 -TT RFC 3 . RT-qPCR results showed that the expression levels of SHISA3 were lower in the lung than in spleen, kidney, muscle and lymph ( P & lt; 0.05), and RFC3 was the lung had a highly significant higher expression level than the heart, liver, spleen, and muscle ( P & lt; 0.01). In conclusion, SHISA3 and RFC3 polymorphisms can be used as genetic markers for improving feed conversion efficiency in Hu sheep.
Type of Medium:
Online Resource
ISSN:
2297-1769
DOI:
10.3389/fvets.2022.1010045
DOI:
10.3389/fvets.2022.1010045.s001
DOI:
10.3389/fvets.2022.1010045.s002
DOI:
10.3389/fvets.2022.1010045.s003
DOI:
10.3389/fvets.2022.1010045.s004
DOI:
10.3389/fvets.2022.1010045.s005
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2834243-4
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