In:
Frontiers in Allergy, Frontiers Media SA, Vol. 2 ( 2021-6-29)
Abstract:
Background: Manifestation of respiratory allergy to American cockroach ( Periplaneta americana ) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization pattern is limited by the lack of Periplaneta allergens widely available for component-resolved diagnostics (CRD). Objective: To evaluate a collection of previously described recombinant Periplaneta allergens for CRD in cockroach allergy. Methods: A panel of nine recombinant Periplanet a allergens (Per a 1–5, 7–10) was generated, purified, and subjected to physicochemical characterization by applying circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), amino acid (AA) analysis, and mass spectrometry (MS). Patients ( n = 117) from India, Korea, Venezuela, and Iran, reporting perennial respiratory indoor allergies with IgE sensitization to cockroach ( P. americana and/or Blattella germanica ), were included. The sensitization profile was monitored by the experimental ImmunoCAP testing. Results: ImmunoCAP testing confirmed IgE sensitization to Periplaneta and/or Blattella extract in 98 of 117 patients ( r = 0.95). Five out of 117 patients were sensitized to only one of the two cockroach species. Within the whole study group, the prevalence of sensitization to individual allergens varied from 4% (Per a 2) to 50% (Per a 9), with the highest IgE values to Per a 9. Patients from four countries displayed different sensitization profiles at which Per a 3 and Per a 9 were identified as major allergens in India and Korea. Periplaneta -derived lipocalin and myosin light chain were characterized as new minor allergens, designated as Per a 4 and Per a 8. Periplaneta extract showed higher diagnostic sensitivity than all individual components combined, suggesting the existence of allergens yet to be discovered. Conclusion: Utilization of a panel of purified Periplaneta allergens revealed highly heterogeneous sensitization patterns and allowed the classification of lipocalin and myosin light chain from Periplaneta as new minor allergens.
Type of Medium:
Online Resource
ISSN:
2673-6101
DOI:
10.3389/falgy.2021.691627
DOI:
10.3389/falgy.2021.691627.s001
DOI:
10.3389/falgy.2021.691627.s002
DOI:
10.3389/falgy.2021.691627.s003
DOI:
10.3389/falgy.2021.691627.s004
DOI:
10.3389/falgy.2021.691627.s005
DOI:
10.3389/falgy.2021.691627.s006
DOI:
10.3389/falgy.2021.691627.s007
DOI:
10.3389/falgy.2021.691627.s008
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
3063831-8
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