In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 7 ( 2023-7-21), p. e0284317-
Abstract:
A total of 24 chromosome-specific fluorescence in situ hybridization probes for interphase nucleus analysis were developed to determine the chromosomal content of individual human invasive cytotrophoblasts derived from in v itro cultured assays. At least 75% of invasive cytotrophoblasts were hyperdiploid and the total number of chromosomes ranged from 47 to 61. The results also demonstrated that these hyperdiploid invasive cytotrophoblasts showed significant heterogeneity. The most copy number gains were observed for chromosomes 13, 14, 15, 19, 21, and 22 with average copy number greater than 2.3. A parallel study using primary invasive cytotrophoblasts also showed a similar trend of copy number changes. Conclusively, 24-chromosome analysis of human non-proliferating cytotrophoblasts (interphase nuclei) was achieved. Hyperdiploidy and chromosomal heterogeneity without endoduplication in invasive cytotrophoblasts may suggest a selective advantage for invasion and short lifespan during normal placental development.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0284317
DOI:
10.1371/journal.pone.0284317.g001
DOI:
10.1371/journal.pone.0284317.g002
DOI:
10.1371/journal.pone.0284317.g003
DOI:
10.1371/journal.pone.0284317.g004
DOI:
10.1371/journal.pone.0284317.g005
DOI:
10.1371/journal.pone.0284317.g006
DOI:
10.1371/journal.pone.0284317.g007
DOI:
10.1371/journal.pone.0284317.t001
DOI:
10.1371/journal.pone.0284317.t002
DOI:
10.1371/journal.pone.0284317.s001
DOI:
10.1371/journal.pone.0284317.s002
DOI:
10.1371/journal.pone.0284317.s003
DOI:
10.1371/journal.pone.0284317.s004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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