In:
Genome, Canadian Science Publishing, Vol. 35, No. 3 ( 1992-06-01), p. 436-442
Abstract:
Synthetic oligonucleotides complementary to simple repetitive DNA sequences were used to detect inter- and intra-specific polymorphisms in a leguminous crop plant (chickpea, Cicer arietinum) and its wild relatives. All the investigated repetitive motifs [(GACA) 4 , (GATA) 4 , (GTG) 5 , (CA) 8 , (TCC) 5 , (GGAT) 4 , and (AGTTT) 4 ] were abundantly present and polymorphic in the chickpea genome. Different probes revealed different levels of variability. Whereas species-specific banding patterns were obtained with the (GTG) 5 probe, other probes revealed differences between accessions, or even individuals. The somatic multilocus patterns were stable for all probes.Key words: genetic polymorphism, simple repetitive sequences, DNA fingerprinting, synthetic oligonucleotide probes.
Type of Medium:
Online Resource
ISSN:
0831-2796
,
1480-3321
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
1992
detail.hit.zdb_id:
2020635-5
SSG:
12
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