In:
Cancer Science, Wiley, Vol. 108, No. 4 ( 2017-04), p. 719-731
Abstract:
Ovarian cancer cells can form spheroids under serum‐free suspension culture conditions. The spheroids, which are enriched in cancer stem cells, can result in tumor dissemination and relapse. To identify new targetable molecules in ovarian cancer spheroids, we investigated the differential expression of genes in spheroids compared with that under monolayer culture conditions by qPCR microarray. We identified that SOX 2 is overexpressed in spheroids. We then proved that SOX 2 expression was increased in successive spheroid generations. Besides, knockdown of SOX 2 expression in SKOV 3 or HO 8910 ovarian cancer spheroid cells decreased spheroid formation, cell proliferation, cell migration, resistance to Cisplatin treatment, tumorigenicity, and the expression of stemness‐related genes and epithelial to mesenchymal transition‐related genes, whereas overexpression of SOX 2 in SKOV 3 or HO 8910 ovarian cancer cells showed the opposite effects. In addition, we found that SOX 2 expression was closely associated with chemo‐resistance and poor prognosis in EOC patients. These results strongly suggest that SOX 2 is required to maintain cancer stem cells in ovarian cancer. Targeting SOX 2 in ovarian cancer may be therapeutically beneficial.
Type of Medium:
Online Resource
ISSN:
1347-9032
,
1349-7006
DOI:
10.1111/cas.2017.108.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2115647-5
detail.hit.zdb_id:
2111204-6
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