In:
Journal of Veterinary Diagnostic Investigation, SAGE Publications, Vol. 11, No. 5 ( 1999-09), p. 391-395
Abstract:
The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera ( n = 30), 2) naturally infected pig sera ( n = 40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to 3 state diagnostic laboratories ( n = 200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the 2 assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.
Type of Medium:
Online Resource
ISSN:
1040-6387
,
1943-4936
DOI:
10.1177/104063879901100501
Language:
English
Publisher:
SAGE Publications
Publication Date:
1999
detail.hit.zdb_id:
2265211-5
SSG:
22
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