In:
Stem Cells Translational Medicine, Oxford University Press (OUP), Vol. 9, No. 12 ( 2020-12-01), p. 1617-1630
Abstract:
The vascular wall stores mesenchymal progenitor cells which are able to induce bone regeneration, via direct and paracrine mechanisms. Although much is known regarding perivascular cell regulation of osteoblasts, their regulation of osteoclasts, and by extension utility in states of high bone resorption, is not known. Here, human perivascular stem cells (PSCs) were used as a means to prevent autograft resorption in a gonadectomy-induced osteoporotic spine fusion model. Furthermore, the paracrine regulation by PSCs of osteoclast formation was evaluated, using coculture, conditioned medium, and purified extracellular vesicles. Results showed that PSCs when mixed with autograft bone induce an increase in osteoblast:osteoclast ratio, promote bone matrix formation, and prevent bone graft resorption. The confluence of these factors resulted in high rates of fusion in an ovariectomized rat lumbar spine fusion model. Application of PSCs was superior across metrics to either the use of unpurified, culture-defined adipose-derived stromal cells or autograft bone alone. Under coculture conditions, PSCs negatively regulated osteoclast formation and did so via secreted, nonvesicular paracrine factors. Total RNA sequencing identified secreted factors overexpressed by PSCs which may explain their negative regulation of graft resorption. In summary, PSCs reduce osteoclast formation and prevent bone graft resorption in high turnover states such as gonadectomy-induced osteoporosis. Significance statement Perivascular progenitor cells exert positive regulatory effects on osteoblasts to heal bones, yet their potential role in osteoclast regulation is not known. It is observed that human perivascular progenitor cells reduce osteoclast formation, thereby preventing bone graft resorption and yielding better outcomes in a preclinical xenograft model. In the future, perivascular stem cells could be used to augment bone grafts, serving as a pro-anabolic, antiosteoclastic stimulus for better outcomes in orthopaedics.
Type of Medium:
Online Resource
ISSN:
2157-6564
,
2157-6580
DOI:
10.1002/sctm.20-0152
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2020
detail.hit.zdb_id:
2642270-0
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