In:
Archives of Medical Science, Termedia Sp. z.o.o.
Abstract:
A single measurement of any biomarker may not reflect its full biological meaning. The kinetics of fibrosis-linked microRNAs and their relationship with extracellular matrix (ECM) fibrosis in dilated cardiomyopathy (DCM) have not been explored. Material and methods We evaluated 70 consecutive DCM patients (48 ±12.1 years, left ventricular ejection fraction 24.4 ±7.4%). All patients underwent right ventricular endomyocardial biopsy in order to quantify ECM fibrosis and measure collagen volume fraction (CVF). Circulating microRNAs (miR-21-5p, miR-29b, miR-30c-5p, and miR-133a-3p) were measured with quantitative polymerase chain reaction (PCR) at baseline and at 3 and 12 months. Results Based on the biopsy results, two groups of patients were identified: with (n = 24, 34.3%) and without (n = 46, 65.7%) ECM fibrosis. Except for a single measurement of miR-29b at 3 months (DCM with fibrosis: 6.03 ±0.72 vs. DCM without fibrosis: 6.4 ±0.75 Cq; p 〈 0.05), baseline, 3- and 12-month kinetics of microRNAs did not differ between the two groups. Moreover, 12-month microRNA kinetics did not differ in patients with new-onset DCM (duration 〈 6 months; n = 35) and chronic DCM ( 〉 6 months; n = 35). Only miR-29 at 3 months correlated with CVF (r = –0.31; p 〈 0.05), whereas other microRNAs did not correlate with CVF either at 3 or at 12 months. Conclusions Regardless of ECM fibrosis status or duration of the disease, 12-month patterns of circulating microRNAs are similar in DCM. Correlations between microRNAs, measured at 3 and 12 months, are lower than expected. In this study, regardless of the time point, circulating microRNAs were not able to differentiate between DCM patients with versus without fibrosis.
Type of Medium:
Online Resource
ISSN:
1734-1922
,
1896-9151
DOI:
10.5114/aoms.2019.89777
Language:
Unknown
Publisher:
Termedia Sp. z.o.o.
Publication Date:
2019
detail.hit.zdb_id:
2203781-0
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