In:
Archives of Medical Science, Termedia Sp. z.o.o.
Kurzfassung:
A single measurement of any biomarker may not reflect its full biological meaning. The kinetics of fibrosis-linked microRNAs and their relationship with extracellular matrix (ECM) fibrosis in dilated cardiomyopathy (DCM) have not been explored. Material and methods We evaluated 70 consecutive DCM patients (48 ±12.1 years, left ventricular ejection fraction 24.4 ±7.4%). All patients underwent right ventricular endomyocardial biopsy in order to quantify ECM fibrosis and measure collagen volume fraction (CVF). Circulating microRNAs (miR-21-5p, miR-29b, miR-30c-5p, and miR-133a-3p) were measured with quantitative polymerase chain reaction (PCR) at baseline and at 3 and 12 months. Results Based on the biopsy results, two groups of patients were identified: with (n = 24, 34.3%) and without (n = 46, 65.7%) ECM fibrosis. Except for a single measurement of miR-29b at 3 months (DCM with fibrosis: 6.03 ±0.72 vs. DCM without fibrosis: 6.4 ±0.75 Cq; p 〈 0.05), baseline, 3- and 12-month kinetics of microRNAs did not differ between the two groups. Moreover, 12-month microRNA kinetics did not differ in patients with new-onset DCM (duration 〈 6 months; n = 35) and chronic DCM ( 〉 6 months; n = 35). Only miR-29 at 3 months correlated with CVF (r = –0.31; p 〈 0.05), whereas other microRNAs did not correlate with CVF either at 3 or at 12 months. Conclusions Regardless of ECM fibrosis status or duration of the disease, 12-month patterns of circulating microRNAs are similar in DCM. Correlations between microRNAs, measured at 3 and 12 months, are lower than expected. In this study, regardless of the time point, circulating microRNAs were not able to differentiate between DCM patients with versus without fibrosis.
Materialart:
Online-Ressource
ISSN:
1734-1922
,
1896-9151
DOI:
10.5114/aoms.2019.89777
Sprache:
Unbekannt
Verlag:
Termedia Sp. z.o.o.
Publikationsdatum:
2019
ZDB Id:
2203781-0
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