In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 3 ( 2021-3-12), p. e0248556-
Abstract:
Eruca sativa Mill. (Brassicaceae) is an important edible vegetable and a potential medicinal plant due to the antibacterial activity of its seed oil. Here, the complete chloroplast (cp) genome of E . sativa was de novo assembled with a combination of long PacBio reads and short Illumina reads. The E . sativa cp genome had a quadripartite structure that was 153,522 bp in size, consisting of one large single-copy region of 83,320 bp and one small single-copy region of 17,786 bp which were separated by two inverted repeat (IRa and IRb) regions of 26,208 bp. This complete cp genome harbored 113 unique genes: 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Forty-nine long repetitive sequences and 69 simple sequence repeats were identified in the E . sativa cp genome. A codon usage analysis of the E . sativa cp genome showed a bias toward codons ending in A/T. The E . sativa cp genome was similar in size, gene composition, and linearity of the structural region when compared with other Brassicaceae cp genomes. Moreover, the analysis of the synonymous (Ks) and non-synonymous (Ka) substitution rates demonstrated that protein-coding genes generally underwent purifying selection pressure, expect ycf1 , ycf2 , and rps12 . A phylogenetic analysis determined that E . sativa is evolutionarily close to important Brassica species, indicating that it may be possible to transfer favorable E . sativa alleles into other Brassica species. Our results will be helpful to advance genetic improvement and breeding of E . sativa , and will provide valuable information for utilizing E . sativa as an important resource to improve other Brassica species.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0248556
DOI:
10.1371/journal.pone.0248556.g001
DOI:
10.1371/journal.pone.0248556.g002
DOI:
10.1371/journal.pone.0248556.g003
DOI:
10.1371/journal.pone.0248556.g004
DOI:
10.1371/journal.pone.0248556.g005
DOI:
10.1371/journal.pone.0248556.g006
DOI:
10.1371/journal.pone.0248556.g007
DOI:
10.1371/journal.pone.0248556.t001
DOI:
10.1371/journal.pone.0248556.t002
DOI:
10.1371/journal.pone.0248556.t003
DOI:
10.1371/journal.pone.0248556.t004
DOI:
10.1371/journal.pone.0248556.t005
DOI:
10.1371/journal.pone.0248556.t006
DOI:
10.1371/journal.pone.0248556.s001
DOI:
10.1371/journal.pone.0248556.s002
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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