In:
HortScience, American Society for Horticultural Science, Vol. 57, No. 4 ( 2022-04), p. 541-547
Abstract:
Nanocrystal cellulose possesses a strong capability to chelate Fe due to its adsorptive properties. Iron deficiency chlorosis (IDC) is a mineral disorder that remarkably weakens pear photosynthesis, causing declines in plant yields and quality. Conventional methods for controlling IDC generally lack efficiency and overuse chemicals. Foliar application of nanocellulose (NC)-Fe chelate (NCFe) provides a new approach to remediate IDC in pear ( Pyrus betulifolia ). In this study, NC was prepared by acidic hydrolysis using 64 wt% H 2 SO 4 at 45 °C for 45 minutes. NCFe was formulated based on the net charge density of NC and ferrous sulfate (FeSO 4 ) solution. The nanoparticle properties were characterized by transmission electron microscopy (TEM), dynamic light scattering, and conductometry. Pyrus betulifolia seedlings were pre-etiolated in an improved Hoagland’s nutrient solution and treated with bicarbonate. Changes in chlorophyll content, active Fe content, and photosynthesis rate in NCFe-treated leaves were determined by SPAD values, spectrophotometry, and photosynthetic apparatus, respectively. Ferritin genes ( PbFER ) and pectin methylesterase genes ( PbPME ) were extracted from leaf tissue, and gene expression profiles were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that NCFe particles maintained a whisker-like morphology; the Z-average hydrodynamic diameter and zeta potential of NCFe measured by dynamic light scattering were 107.4 ± 3.0 nm and −9.7 ± 0.4 mV, respectively. When NCFe was prepared at a mixing ratio of 1:3000, the total chlorophyll content, active Fe content, and net photosynthetic rate of plant leaves were significantly enhanced by 23.8%, 65.9%, and 40.4% after 72 hours of treatment, respectively, compared with FeSO 4 spraying. Importantly, NCFe treatment also significantly downregulated the expression of PbPME and upregulated the expression of PbFER , which are key genes regulating the active Fe content.
Type of Medium:
Online Resource
ISSN:
0018-5345
,
2327-9834
DOI:
10.21273/HORTSCI16404-21
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
2022
detail.hit.zdb_id:
2040198-X
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