In:
Biology of the Cell, Wiley, Vol. 87, No. 1-2 ( 1996-01), p. 37-43
Abstract:
Summary— A three‐dimensional reconstruction analysis of localization of phosphodiester and phosphorothioate oligonucleotide antisense to type‐I plasminogen activator inhibitor (PAI‐1) mRNA within endothelial cells is described. When EA.hy 926 cells were incubated with fluorescently labelled phosphodiester (PO‐16) or phosphorothioate (PS‐16) oligonucleotides at low, not cytotoxical concentrations, the relative brightness composition of the images of the particular samples was much higher for PS‐16 than PO‐16 and dependent upon the extracellular concentration and the incubation time. The 3‐D reconstructions based on the series of optical sections of the samples, spaced every 1.5gμm, showed the punctuate accumulation of the oligonucleotides and a striking difference in a spatial distribution between PO‐16 and PS‐16 within the cytoplasm. Even after 24 h incubation of endothelial cells with 2.5 μM of PO‐16 and PS‐16 oligonucleotides, there was a predominant oligonucleotide localization within the cytoplasm and only traces of oligonucleotides could be seen in the cell nucleus and/or perinuclear organelles.
Type of Medium:
Online Resource
ISSN:
0248-4900
,
1768-322X
DOI:
10.1111/boc.1996.87.issue-1-2
DOI:
10.1111/j.1768-322X.1996.tb00964.x
Language:
English
Publisher:
Wiley
Publication Date:
1996
detail.hit.zdb_id:
2011750-4
SSG:
12
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