In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_3 ( 2014-03-01), p. 812-818
Abstract:
A novel, anaerobic filamentous bacterium, strain MO-CFX1 T , was isolated from a methanogenic community, which was originally established from subseafloor sediments collected from off the Shimokita Peninsula, Japan. Cells were non-spore-forming, non-motile, Gram-stain-negative and filamentous. The filaments were longer than 10 µm and 130–150 nm in width. Growth of the strain was observed at 10–37 °C (optimum 25–30 °C), at pH 5.5–8.5 (optimum pH 7.0) and in 0–50 g NaCl l −1 (optimum 15 g NaCl l −1 ). The strain was able to grow with a number of carbohydrates in the presence of yeast extract. The major cellular fatty acids were monounsaturated C 18 : 1 ω9, C 16 : 1 ω7 and saturated C 18 : 0 and C 16 : 0 . The intact polar lipids of the strain were dominated by diacylglyceride and sphingolipid core lipid structures with monoglycosidic, mixed phosphomonoglycosidic and fatty-acid-modified monoglycosidic polar head groups. The G+C content of the genomic DNA was 52.4 mol%. Based on the comparative 16S rRNA gene sequence analysis, strain MO-CFX1 T was affiliated with the class Anaerolineae within the phylum Chloroflexi and was most closely related to Leptolinea tardivitalis YMTK-2 T (sequence identity of 91.0 %). Based on phenotypic and genetic properties of the novel isolate, we propose a novel species representing a new genus Pelolinea submarina gen. nov., sp. nov., for strain MO-CFX1 T ( = JCM 17238 T , = KCTC 5975 T ). This is the first formal description, to our knowledge, of an isolate of the phylum Chloroflexi from the deep-sea sedimentary environment.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.057547-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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