In:
Functional Plant Biology, CSIRO Publishing, Vol. 27, No. 9 ( 2000), p. 787-
Abstract:
This paper originates from a presentation at the International Conference on Assimilate Transport and Partitioning, Newcastle, NSW, August 1999
The entire 3.7 kbp 5 & acute;-upstream region (–2840 to +886) from
the translational start codon of NADH–glutamate synthase (NADH–GOGAT, EC 1.4.1.14) gene from rice
(Oryza sativa L.) or the region sequentially deleted from the 5 & acute;-end was fused with the β−glucuronidase (GUS)
reporter gene. The chimeric gene was introduced into calli derived from rice scutellum via Agrobacterium tumefaciens-mediated
transformation and tissue-specific GUS activity determined in T0 generations. When the entire region was fused, GUS activity was detected in vascular
bundles of the developing leaf blade and in dorsal and lateral vascular bundles of developing grains. This corresponds with our previous
immunodetection of NADH–GOGAT protein (Hayakawa et al., Planta
193, 455–460, 1994). A series of deletion experiments showed that a 149-nucleotide region between –142 and +7
was essential for promoter activity in the NADH–GOGAT gene.
Type of Medium:
Online Resource
ISSN:
1445-4408
Language:
English
Publisher:
CSIRO Publishing
Publication Date:
2000
SSG:
12
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