In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-6-9)
Abstract:
Scutellaria baicalensis Georgi. (Chinese skullcap or Huang-qin) is an extremely crucial medicinal plant in the Labiate family, and the color of its flowers naturally appears purple. However, during the long-term cultivation of S . baicalensis , very few plants of S . baicalensis also present white and purple-red flower colors under the same ecological conditions. However, the complex metabolic and transcriptional networks underlying color formation in white, purple-red, and purple flowers of S . baicalensis remain largely unclarified. To gain an insight into this issue, we conducted transcriptome and metabolomic profiling to elucidate the anthocyanin synthesis metabolic pathway in the flowers of S . baicalensis , and to identify the differentially expressed candidate genes potentially involved in the biosynthesis of anthocyanins. The results showed that 15 anthocyanins were identified, among which cyanidin 3-rutinoside and delphin chloride were the primary anthocyanins, and accumulation was significantly related to the flower color changes of S . baicalensis . Furthermore, the down-regulation of SbDFR ( Sb02g31040 ) reduced the anthocyanin levels in the flowers of S . baicalensis . The differential expression of the Sb3GT ( Sb07g04780 and Sb01g72290 ) gene in purple and purple-red flowers affected anthocyanin accumulation, suggesting that anthocyanin levels were closely associated with the expression of SbDFR and Sb3GT , which play important roles in regulating the anthocyanin biosynthesis process of S . baicalensis flowers. Transcriptomic analysis revealed that transcription factors WRKY, bHLH, and NAC were also highly correlated with anthocyanin accumulation, especially for NAC35, which positively regulated SbDFR ( Sb02g31040 ) gene expression and modulated anthocyanin biosynthesis in flower color variation of S . baicalensis . Overall, this study presents the first experimental evidence for the metabolomic and transcriptomic profiles of S . baicalensis in response to flower coloration, which provides a foundation for dynamic metabolic engineering and plant breeding, and to understand floral evolution in S . baicalensis plants.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2022.884957
DOI:
10.3389/fpls.2022.884957.s001
DOI:
10.3389/fpls.2022.884957.s002
DOI:
10.3389/fpls.2022.884957.s003
DOI:
10.3389/fpls.2022.884957.s004
DOI:
10.3389/fpls.2022.884957.s005
DOI:
10.3389/fpls.2022.884957.s006
DOI:
10.3389/fpls.2022.884957.s007
DOI:
10.3389/fpls.2022.884957.s008
DOI:
10.3389/fpls.2022.884957.s009
DOI:
10.3389/fpls.2022.884957.s010
DOI:
10.3389/fpls.2022.884957.s011
DOI:
10.3389/fpls.2022.884957.s012
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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