In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 73, No. 5 ( 2023-05-12)
Abstract:
A taxonomic study was carried out on strain yzlin-01 T , isolated from Dongshan Island seawater. The bacterium was Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, and motile by polar flagella. Growth was observed at temperatures of 10–40 °C, at salinities of 0.5–18 %, and at pH of 6–10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain yzlin-01 T belonged to the genus Halomonas , with the highest sequence similarity to Halomonas malpeensis YU-PRIM-29 T (96.7 %), followed by Halomonas johnsoniae T68687 T (96.4 %) and Halomonas gomseomensis M12 T (96.4 %), and other species of the genus Halomonas (93.4–96.3 %). The ANI and digital DNA–DNA hybridization estimate values between strain yzlin-01 T and the closest type strain Halomonas malpeensis YU-PRIM-29 T were 77.44 and 21.6 %, respectively. The principal fatty acids were summed feature 8 (consisting of C 18 : 1 ω7 c and/or C 18 : 1 ω6 c; 55.7 %), C 16 : 0 (20.6 %), C 12 : 0 3-OH (6.8 %), summed feature 3 (consisting of C 16 : 1 ω7 c and/or C 16 : 1 ω6 c; 5.1 %). The G+C content of the chromosomal DNA was 60.0 mol %. The respiratory quinone was identified as Q-9 (100 %). Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, and three unidentified phospholipids were present. Combined genotypic and phenotypic data suggest that strain yzlin-01 T represents a novel species within the genus Halomonas , for which the name Halomonas dongshanensis sp. nov. is proposed, with the type strain yzlin-01 T (=GDMCC 1.3202 T =KCTC 92467 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005830
Language:
English
Publisher:
Microbiology Society
Publication Date:
2023
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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