In:
The Scientific World Journal, Hindawi Limited, Vol. 2014 ( 2014), p. 1-8
Abstract:
Phyla nodiflora is a creeping perennial herb, widely distributed in the most tropical and subtropical regions. It has been used as a folk medicine, herbal beverage, or folk cosmetic. For these usages, the development of a chemical quality control method of this plant is necessary. In the present study, ten compounds, namely, 3,7,4′,5′-tetrahydroxy-3′-methoxyflavone ( 1 ), nodifloretin ( 2 ), 4′-hydroxywogonin ( 3 ), onopordin ( 4 ), cirsiliol ( 5 ), 5,7,8,4′-tetrahydroxy-3′-methoxyflavone ( 6 ), eupafolin ( 7 ), hispidulin ( 8 ), larycitrin ( 9 ), and β -sitosterol were isolated from the methanolic extract of the aerial part of P. nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature. The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems. Compounds 4 , 5 , and 7 showed strong antioxidant activity. To control the quality of P. nodiflora , a simple and reliable method of high-performance liquid chromatography combined with ultraviolet detector (HPLC-UV) was established for both the fingerprint analysis and the quantitative determination of two selected active compounds, onopordin ( 4 ) and eupafolin ( 7 ). Statistical analysis of the obtained data demonstrated that our method achieved the desired linearity, precision, and accuracy. The results indicated that the developed method can be used as a quality evaluation method for PNM.
Type of Medium:
Online Resource
ISSN:
2356-6140
,
1537-744X
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2014
detail.hit.zdb_id:
2075968-X
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