In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 8 ( 2023-8-23), p. e0289272-
Abstract:
Quercus mongolica is a common landscape, afforestation, and construction timber species in northern China with high ecological, economic, and ornamental value. Leaf senescence is a complex process that has important implications for plant growth and development. To explore changes of metabolites during the ageing of Quercus mongolica leaves, we investigated physiological responses and metabolite composition in ageing leaves harvested from 15–20-year-old Quercus mongolica . Leaf samples of Q . mongolica were collected when they were still green (at maturity) (stage 1), during early senescence (stage 2), and during late senescence (stage 3). These leaves were then subjected to physiological index and metabolome sequencing analyses. The physiological analysis showed that the leaves of Q . mongolica changed from green to yellow during senescence, which induced significant accumulation of soluble sugar and significant reductions in the concentration of soluble protein and chlorophyll. Peroxidase and catalase were the main antioxidant enzymes mitigating leaf senescence. Metabolomic analysis identified 797 metabolites during leaf senescence. Compared to stage 1, 70 differential metabolites were screened in stage 2 and 72 were screened in stage 3. Differential metabolites in the two senescent stages were principally enriched in amino acid metabolism, lipid metabolism and secondary metabolite biosynthesis. The contents of N-oleoylethanolamine and N, N-dimethylglycine were significantly increased only in stage 2, while the contents of trifolin, astragalin, valine, isoleucine, leucine, and citric acid were significantly increased only in stage 3. Histidine, homoserine, tryptophan, tyrosine, phenylalanine, proline, norleucine, N-glycyl-L-leucine, linoleic acid, linolenic acid, gallic acid, 3-indoleacrylic acid, 3-amino-2-naphthoic acid, 3-hydroxy-3-methylpentane-1,5-dioic acid, 2,3,4-trihydroxybenzoic acid, trifolin, astragalin, DL-2-aminoadipic acid, pinoresinol dimethyl ether, dimethylmatairesinol, and lysophosphatidylcholine increased during both stage 2 and stage 3. Increasing contents of these metabolites may constitute the main mechanism by which Q . mongolica leaves adapt to senescence.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0289272
DOI:
10.1371/journal.pone.0289272.g001
DOI:
10.1371/journal.pone.0289272.g002
DOI:
10.1371/journal.pone.0289272.g003
DOI:
10.1371/journal.pone.0289272.g004
DOI:
10.1371/journal.pone.0289272.g005
DOI:
10.1371/journal.pone.0289272.g006
DOI:
10.1371/journal.pone.0289272.g007
DOI:
10.1371/journal.pone.0289272.g008
DOI:
10.1371/journal.pone.0289272.t001
DOI:
10.1371/journal.pone.0289272.t002
DOI:
10.1371/journal.pone.0289272.s001
DOI:
10.1371/journal.pone.0289272.s002
DOI:
10.1371/journal.pone.0289272.s003
DOI:
10.1371/journal.pone.0289272.s004
DOI:
10.1371/journal.pone.0289272.s005
DOI:
10.1371/journal.pone.0289272.s006
DOI:
10.1371/journal.pone.0289272.s007
DOI:
10.1371/journal.pone.0289272.s008
DOI:
10.1371/journal.pone.0289272.s009
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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