In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 61, No. 4 ( 2011-04-01), p. 888-893
Abstract:
A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, catalase-positive, oxidase-negative, endospore-forming, motile, rod-shaped, aerobic bacterium, designated strain JSM 082006 T , was isolated from an oyster collected from Naozhou Island in the South China Sea. The isolate grew in 0–18 % (w/v) NaCl (optimum, 0.5–4.0 %), at pH 6.0–10.5 (optimum, pH 8.0) and at 15–45 °C (optimum, 30 °C). meso -Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and C 16 : 0 . Strain JSM 082006 T contained MK-7 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The genomic DNA G+C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 082006 T should be assigned to the genus Bacillus and that it was most closely related to the type strains of Bacillus barbaricus (sequence similarity 99.1 %) and Bacillus arsenicus (97.5 %), followed by those of Bacillus rigui (96.6 %) and Bacillus solisalsi (96.1 %). Phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data support the view that strain JSM 082006 T represents a novel species of the genus Bacillus , for which the name Bacillus nanhaiensis sp. nov. is proposed; the type strain is JSM 082006 T ( = DSM 23009 T = KCTC 13712 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.022889-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2011
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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