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  • 1
    In: Journal of Clinical Microbiology, American Society for Microbiology, Vol. 45, No. 11 ( 2007-11), p. 3791-3794
    Abstract: Spoligotyping was performed to study the population structure of Mycobacterium tuberculosis complex strains ( n = 224) from Bangladesh. Strains were split into principal genetic group 1 (PGG 1 [75.0%]) and PGG 2 and 3 (25%). Forty-nine strains with a new spoligotype signature and considered as south or southeast Asian-linked emerging clones were designated as “Matlab type.”
    Type of Medium: Online Resource
    ISSN: 0095-1137 , 1098-660X
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2007
    detail.hit.zdb_id: 1498353-9
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Bangladesh Academy of Sciences ; 2023
    In:  Dhaka University Journal of Biological Sciences Vol. 31, No. 2 ( 2023-02-02), p. 309-319
    In: Dhaka University Journal of Biological Sciences, Bangladesh Academy of Sciences, Vol. 31, No. 2 ( 2023-02-02), p. 309-319
    Abstract: A serious departure from the norm in taking classes has been a regular phenomenon in the University of Dhaka in recent years. The present study aimed to investigate perceptions, reactions, and grievances of students towards class cancellation, class rescheduling, inadequate classes, and classes for less than duration at the University of Dhaka. Data were collected online from 522 students (51.5% male and 48.5% female, mean age = 21.74 years) of the university using a mixed-method design. The quantitative data were analyzed by t-test, Chi-square test, and Pearson product-moment correlation coefficients in SPSS version 24. The results of the t-test demonstrated no significant gender differences in perception towards the main variables. The Chi-square test reveals that the frequencies of occurrences of these phenomena significantly differ among the faculties of four hierarchical positions. The content analysis of the qualitative data revealed 12 major themes namely the four factors across academic units, the favorable impact of these phenomena, increased absenteeism, the sources of stress, anxiety, and frustration, lack of priority, teachers’ disinterest in teaching and learning, a sense of punctuality and responsibility, mutual understanding, timetable of the rescheduled classes, teacher’s accountability and students’ feedback, introducing training for teachers, and the role of authority. The findings of the study suggest a clear lack of administrative control over the teaching behavior of the faculties. The study recommends that the university authority should immediately address these issues to redress the grievances of the students and thereby ensure a quality culture in teaching, learning and research. Dhaka Univ. J. Biol. Sci. 31(2): 309-319, 2022 (July)
    Type of Medium: Online Resource
    ISSN: 2408-8501 , 1021-2787
    Language: Unknown
    Publisher: Bangladesh Academy of Sciences
    Publication Date: 2023
    detail.hit.zdb_id: 2771701-X
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  • 3
    Online Resource
    Online Resource
    Bangladesh Academy of Sciences ; 1970
    In:  Stamford Journal of Microbiology Vol. 1, No. 1 ( 1970-01-01), p. 31-36
    In: Stamford Journal of Microbiology, Bangladesh Academy of Sciences, Vol. 1, No. 1 ( 1970-01-01), p. 31-36
    Abstract: Various types of cooked food samples were collected from six different hospitals within the city of Dhaka and they were analyzed for the presence (if any) of total aerobic count (TAC), total coliform count (TCC) and total staphylococcal count (TSA) in order to determine the levels of contamination and to relate these findings to the hygiene practice of the food handlers. According to Gulf standard for microbiological criteria for foodstuff, all of the food samples exceeded the acceptable total aerobic count limit of 5 x 105 cfu/g while 4 out of 6 samples exceeded coliform count limit of 1x102 cfu/g. The total coliform counts were found to be the highest in the fish (1.6 x 107 cfu/g) and egg (2.2 x 106 cfu/g) curry samples of hospital 1 and hospital 4, respectively and were the lowest in the fish curry (2 x 103 cfu/g) of hospital 4. Staphylococcus aureus was found in all of the food samples with the highest occurrence (too numerous to count) in Dal and Rice samples from hospitals 3 and 6, respectively. On Xylose Lysine Deoxycholate (XLD) agar, no black centered colony but many colorless colonies were found which primarily indicated the absence of Salmonella spp. in those samples. In order to identify pathogenic microorganisms from food samples, a series of conventional biochemical tests were performed with 23 randomly selected isolates from MacConkey, XLD, MSA agar plates. The isolates were presumptively identified as Escherichia coli, Staphylococcus aureus, Shigella spp. and Pseudomonas spp. etc. The antibiotic susceptibility test was performed with eleven selected isolates using six commonly prescribed antibiotics (ampicillin, tetracyclines, ciprofloxacin, vancomycin, gentamicin and azithromycin). The results showed that six isolates were resistant to vancomycin, two isolates were multidrug resistant and one isolate was intermediately resistant to azithromycin. All the isolates were found to be sensitive to ciprofloxacin, and gentamicin. Based on the data, it can be suggested that adequate hygiene practices are required after cooking the foods and before serving them as they reconsider.DOI: http://dx.doi.org/10.3329/sjm.v1i1.9100  Stamford Journal of Microbiology, Vol.1(1), July 2011, p.31-36  
    Type of Medium: Online Resource
    ISSN: 2408-8846 , 2074-5346
    Language: Unknown
    Publisher: Bangladesh Academy of Sciences
    Publication Date: 1970
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  • 4
    Online Resource
    Online Resource
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 22, No. 1 ( 2020-12-26), p. 167-
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 1 ( 2020-12-26), p. 167-
    Abstract: The early signaling events involved in oxidant recognition and triggering of oxidant-specific defense mechanisms to counteract oxidative stress still remain largely elusive. Our discovery driven comparative proteomics analysis revealed unique early signaling response of the yeast Saccharomyces cerevisiae on the proteome level to oxidants with a different mechanism of action as early as 3 min after treatment with four oxidants, namely H2O2, cumene hydroperoxide (CHP), and menadione and diamide, when protein abundances were compared using label-free quantification relying on a high-resolution mass analyzer (Orbitrap). We identified significant regulation of 196 proteins in response to H2O2, 569 proteins in response to CHP, 369 proteins in response to menadione and 207 proteins in response to diamide. Only 17 proteins were common across all treatments, but several more proteins were shared between two or three oxidants. Pathway analyses revealed that each oxidant triggered a unique signaling mechanism associated with cell survival and repair. Signaling pathways mostly regulated by oxidants were Ran, TOR, Rho, and eIF2. Furthermore, each oxidant regulated these pathways in a unique way indicating specificity of response to oxidants having different modes of action. We hypothesize that interplay of these signaling pathways may be important in recognizing different oxidants to trigger different downstream MAPK signaling cascades and to induce specific responses.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 5
    In: Genetics, Oxford University Press (OUP), Vol. 200, No. 1 ( 2015-05-01), p. 167-184
    Abstract: Diet is a central environmental factor that contributes to the phenotype and physiology of individuals. At the root of many human health issues is the excess of calorie intake relative to calorie expenditure. For example, the increasing amount of dietary sugars in the human diet is contributing to the rise of obesity and type 2 diabetes. Individuals with obesity and type 2 diabetes have compromised oxygen delivery, and thus it is of interest to investigate the impact a high-sugar diet has on oxygen deprivation responses. By utilizing the Caenorhabditis elegans genetic model system, which is anoxia tolerant, we determined that a glucose-supplemented diet negatively impacts responses to anoxia and that the insulin-like signaling pathway, through fatty acid and ceramide synthesis, modulates anoxia survival. Additionally, a glucose-supplemented diet alters lipid localization and initiates a positive chemotaxis response. Use of RNA-sequencing analysis to compare gene expression responses in animals fed either a standard or glucose-supplemented diet revealed that glucose impacts the expression of genes involved with multiple cellular processes including lipid and carbohydrate metabolism, stress responses, cell division, and extracellular functions. Several of the genes we identified show homology to human genes that are differentially regulated in response to obesity or type 2 diabetes, suggesting that there may be conserved gene expression responses between C. elegans fed a glucose-supplemented diet and a diabetic and/or obesity state observed in humans. These findings support the utility of the C. elegans model for understanding the molecular mechanisms regulating dietary-induced metabolic diseases.
    Type of Medium: Online Resource
    ISSN: 1943-2631
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2015
    detail.hit.zdb_id: 1477228-0
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Bangladesh Academy of Sciences ; 1970
    In:  Stamford Journal of Pharmaceutical Sciences Vol. 1, No. 1 ( 1970-01-01), p. 69-75
    In: Stamford Journal of Pharmaceutical Sciences, Bangladesh Academy of Sciences, Vol. 1, No. 1 ( 1970-01-01), p. 69-75
    Abstract: Cholera caused by toxigenic Vibrio cholerae is a major public health problem confronting developing countries, where outbreaks occur in a regular seasonal pattern and are particularly associated with poverty and poor sanitation. It is generally accepted that seven distinct pandemics of cholera have occurred since the onset of the first pandemic in 1817. Again Vibrio cholerae is capable of surviving in aquatic environments for extended periods and is considered as autochthonous species in estuarine and brackish waters. Therefore, the present study was designed to isolate V. cholerae from natural environmental samples subsequently identified by conventional and molecular biological techniques. A total number of 10 isolates were included randomly in this study based on their initial identification. The serotypes of the isolates were determined by serological test (slide agglutination) and the number of serotypes O1, O139 and non-O1/O139 were 3, 2 and 5 respectively which were reconfirmed by PCR method. Finally, the toxigenicity of the isolates was analyzed by multiplex PCR method and five (5) isolates were found to contain the ctx gene, the major virulence factor of V. cholerae. Key Words: Vibrio cholerae, Simplex PCR, Multiplex PCR, Serotypes, Toxigenicity.   doi:10.3329/sjps.v1i1.1811 S. J. Pharm. Sci. 1(1 & 2): 69-75
    Type of Medium: Online Resource
    ISSN: 1999-7108
    Language: Unknown
    Publisher: Bangladesh Academy of Sciences
    Publication Date: 1970
    detail.hit.zdb_id: 2491225-6
    SSG: 15,3
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  • 7
    In: Pharmaceutics, MDPI AG, Vol. 12, No. 2 ( 2020-01-27), p. 101-
    Abstract: To facilitate the development of broad-spectrum retina neuroprotectants that can be delivered through topical dosage forms, this proteomics study focused on analyzing target engagements through the identification of functional protein networks impacted after delivery of 17β-estradiol in eye drops. Specifically, the retinae of ovariectomized Brown Norway rats treated with daily eye drops of 17β-estradiol for three weeks were compared to those of vehicle-treated ovariectomized control animals. We searched the acquired raw data against a composite protein sequence database by using Mascot, as well as employed label-free quantification to detect changes in protein abundances. Our investigation using rigorous validation criteria revealed 331 estrogen-regulated proteins in the rat retina (158 were up-regulated, while 173 were down-regulated by 17β-estradiol delivered in eye drops). Comprehensive pathway analyses indicate that these proteins are relevant overall to nervous system development and function, tissue development, organ development, as well as visual system development and function. We also present 18 protein networks with associated canonical pathways showing the effects of treatments for the detailed analyses of target engagements regarding potential application of estrogens as topically delivered broad-spectrum retina neuroprotectants. Profound impact on crystallins is discussed as one of the plausible neuroprotective mechanisms.
    Type of Medium: Online Resource
    ISSN: 1999-4923
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2527217-2
    SSG: 15,3
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  • 8
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 4 ( 2021-02-08), p. 1686-
    Abstract: The widely used rat uterotrophic assay to assess known and potential estrogenic compounds only considers uterine weight gain as endpoint measurement. To complement this method with an advanced technology that reveals molecular targets, we analyzed changes in protein expression using label-free quantitative proteomics by nanoflow liquid chromatography coupled with high-resolution mass spectrometry and tandem mass spectrometry from uterine protein extracts of ovariectomized rats after daily 17β-estradiol exposure for five days in comparison with those of vehicle-treated control animals. Our discovery-driven study revealed 165 uterine proteins significantly regulated by estrogen treatment and mapped by pathway analyses. Estrogen-regulated proteins represented cell death, survival and development, cellular growth and proliferation, and protein synthesis as top molecular and cellular functions, and a network found with the presence of nuclear estrogen receptor(s) as a prominent molecular node confirmed the relevance of our findings to hormone-associated events. An exploratory application of targeted proteomics to bisphenol A as a well-known example of an estrogenic endocrine disruptor is also presented. Overall, the results of this study have demonstrated the power of combining untargeted and targeted quantitative proteomic strategies to identify and verify candidate molecular markers for the evaluation of endocrine-disrupting chemicals to complement a conventional bioassay.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 9
    In: Journal of Molecular Structure, Elsevier BV, Vol. 1130 ( 2017-02), p. 994-1000
    Type of Medium: Online Resource
    ISSN: 0022-2860
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2017
    detail.hit.zdb_id: 1491504-2
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  • 10
    In: BMC Microbiology, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2006-12)
    Abstract: The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member of the CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping is the widely used PCR-based reverse-hybridization blotting technique that assays the genetic diversity of this locus and is useful both for clinical laboratory, molecular epidemiology, evolutionary and population genetics. It is easy, robust, cheap, and produces highly diverse portable numerical results, as the result of the combination of (1) Unique Events Polymorphism (UEP) (2) Insertion-Sequence-mediated genetic recombination. Genetic convergence, although rare, was also previously demonstrated. Three previous international spoligotype databases had partly revealed the global and local geographical structures of MTC bacilli populations, however, there was a need for the release of a new, more representative and extended, international spoligotyping database. Results The fourth international spoligotyping database, SpolDB4, describes 1939 shared-types (STs) representative of a total of 39,295 strains from 122 countries, which are tentatively classified into 62 clades/lineages using a mixed expert-based and bioinformatical approach. The SpolDB4 update adds 26 new potentially phylogeographically-specific MTC genotype families. It provides a clearer picture of the current MTC genomes diversity as well as on the relationships between the genetic attributes investigated (spoligotypes) and the infra-species classification and evolutionary history of the species. Indeed, an independent Naïve-Bayes mixture-model analysis has validated main of the previous supervised SpolDB3 classification results, confirming the usefulness of both supervised and unsupervised models as an approach to understand MTC population structure. Updated results on the epidemiological status of spoligotypes, as well as genetic prevalence maps on six main lineages are also shown. Our results suggests the existence of fine geographical genetic clines within MTC populations, that could mirror the passed and present Homo sapiens sapiens demographical and mycobacterial co-evolutionary history whose structure could be further reconstructed and modelled, thereby providing a large-scale conceptual framework of the global TB Epidemiologic Network. Conclusion Our results broaden the knowledge of the global phylogeography of the MTC complex. SpolDB4 should be a very useful tool to better define the identity of a given MTC clinical isolate, and to better analyze the links between its current spreading and previous evolutionary history. The building and mining of extended MTC polymorphic genetic databases is in progress.
    Type of Medium: Online Resource
    ISSN: 1471-2180
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2006
    detail.hit.zdb_id: 2041505-9
    SSG: 12
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