In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 5 ( 2023-5-2), p. e0272674-
Abstract:
Decades of research on marine N 2 fixation focused on Trichodesmium , which are generally free-living cyanobacteria, but in recent years the endosymbiotic cyanobacterium Candidatus Atelocyanobacterium thalassa (UCYN-A) has received increasing attention. However, few studies have shed light on the influence of the host versus the habitat on UCYN-A N 2 fixation and overall metabolism. Here we compared transcriptomes from natural populations of UCYN-A from oligotrophic open-ocean versus nutrient-rich coastal waters, using a microarray that targets the full genomes of UCYN-A1 and UCYN-A2 and known genes for UCYN-A3. We found that UCYN-A2, usually regarded as adapted to coastal environments, was transcriptionally very active in the open ocean and appeared to be less impacted by habitat change than UCYN-A1. Moreover, for genes with 24 h periodic expression we observed strong but inverse correlations among UCYN-A1, A2, and A3 to oxygen and chlorophyll, which suggests distinct host-symbiont relationships. Across habitats and sublineages, genes for N 2 fixation and energy production had high transcript levels, and, intriguingly, were among the minority of genes that kept the same schedule of diel expression. This might indicate different regulatory mechanisms for genes that are critical to the symbiosis for the exchange of nitrogen for carbon from the host. Our results underscore the importance of N 2 fixation in UCYN-A symbioses across habitats, with consequences for community interactions and global biogeochemical cycles.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0272674
DOI:
10.1371/journal.pone.0272674.g001
DOI:
10.1371/journal.pone.0272674.g002
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10.1371/journal.pone.0272674.g003
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10.1371/journal.pone.0272674.g004
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10.1371/journal.pone.0272674.s001
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10.1371/journal.pone.0272674.s002
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10.1371/journal.pone.0272674.s003
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10.1371/journal.pone.0272674.s004
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10.1371/journal.pone.0272674.s005
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10.1371/journal.pone.0272674.s006
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10.1371/journal.pone.0272674.s007
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10.1371/journal.pone.0272674.s008
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10.1371/journal.pone.0272674.s009
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10.1371/journal.pone.0272674.s010
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10.1371/journal.pone.0272674.s011
DOI:
10.1371/journal.pone.0272674.s012
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10.1371/journal.pone.0272674.s013
DOI:
10.1371/journal.pone.0272674.s014
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10.1371/journal.pone.0272674.s015
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10.1371/journal.pone.0272674.s016
DOI:
10.1371/journal.pone.0272674.s017
DOI:
10.1371/journal.pone.0272674.s018
DOI:
10.1371/journal.pone.0272674.s019
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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