In:
North American Journal of Aquaculture, Wiley, Vol. 80, No. 2 ( 2018-04), p. 119-127
Abstract:
Nitric oxide synthase ( NOS ) plays important roles in the immune system of animals. As an important marine bivalve cultured in south China, the noble scallop Chlamys nobilis is subject to diseases of various kinds, leading to decrease harvest. To investigate whether NOS in the noble scallop (hereafter, Cn NOS ) plays a significant role in immune defense, the full‐length open‐reading frame ( ORF ) of the Cn NOS gene was cloned, and its expression levels in different tissues were determined. The complementary DNA nucleotide sequence of Cn NOS encompassed 5,345 bp, with a full‐length ORF of 4,647 bp. The gene consisted of the typical NOS structure, including an NOS domain, a flavodoxin 1 domain, a flavin adenine dinucleotide binding domain, and a conservative nicotinamide adenine dinucleotide binding domain. Subsequently, transcript levels of Cn NOS in hemocytes were determined by real‐time quantitative reverse transcription PCR , indicating that Cn NOS expression was significantly up‐regulated in noble scallops injected with different immunostimulants, including lipopolysaccharides; the synthetic double‐stranded RNA , polyinosinic: polycytidylic acid; and Vibrio parahaemolyticus . The present results suggest that the Cn NOS might play a crucial role in the noble scallop's defense against exogenous stimuli.
Type of Medium:
Online Resource
ISSN:
1522-2055
,
1548-8454
DOI:
10.1002/naaq.2018.80.issue-2
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2192450-8
detail.hit.zdb_id:
1482260-X
SSG:
21,3
SSG:
12
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