In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 14, No. 11 ( 2020-11-30), p. e0008873-
Abstract:
Bungarus multicinctus is the most venomous snake distributed in China and neighboring countries of Myanmar, Laos, north Vietnam and Thailand. The high mortality rate of B . multicinctus envenomation is attributed to the lethal components of α-, β-, γ- and κ- bungarotoxins contained in the venom. Although anti- B . multicinctus sera were produced in Shanghai, Taiwan and Vietnam, the most widely clinic used product was term as B . multicinctus antivenin and manufactured by Shanghai Serum Bio-technology Co. Ltd. In the present investigation, high purity α-, β- and γ-bungarotoxins were separately isolated from B . multicinctus crude venom. Rabbit anti- α-, β- and γ-bungarotoxin antisera were prepared by common methods, respectively. LD 50 values of α-, β- and γ-bungarotoxins were systematically determined via three administration pathways (intraperitoneal, intramuscular and intravenous injections) in Kunming mice. LD 50 values of β-bungarotoxin were closely related with injection routines but those of both α- and γ-bungarotoxins were not dependent on the injection routines. Commercial B . multicinctus antivenin showed strong immunoreaction with high molecular weight fractions of the B . multicinctus but weakly recognized low molecular weight fractions like α- and γ-bungarotoxins. Although B . multicinctus antivenin showed immunoreaction with high molecular weight fractions of Bungarus fasciatus , Naja atra , Ophiophagus hannah venoms but the antivenin only demonstrated animal protection efficacy against O . hannah venom. These results indicated that the high molecular weight fractions of the O . hannah played an important role in venom lethality but those of B . fasciatus and N . atra did not have such a role.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0008873
DOI:
10.1371/journal.pntd.0008873.g001
DOI:
10.1371/journal.pntd.0008873.g002
DOI:
10.1371/journal.pntd.0008873.g003
DOI:
10.1371/journal.pntd.0008873.g004
DOI:
10.1371/journal.pntd.0008873.t001
DOI:
10.1371/journal.pntd.0008873.t002
DOI:
10.1371/journal.pntd.0008873.t003
DOI:
10.1371/journal.pntd.0008873.s001
DOI:
10.1371/journal.pntd.0008873.s002
DOI:
10.1371/journal.pntd.0008873.s003
DOI:
10.1371/journal.pntd.0008873.s004
DOI:
10.1371/journal.pntd.0008873.s005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2429704-5
Bookmarklink