In:
Frontiers in Genetics, Frontiers Media SA, Vol. 12 ( 2021-7-8)
Abstract:
Brucellosis is a zoonotic disease and a major public health problem. However, the genetic mechanism of brucellosis in sheep remains unclear. In this study, serum samples were collected from 6,358 sheep from the F2 population (Dorper sheep ♂ × Hu sheep ♀), and antibody levels were continuously measured at 14 days and 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 months after administration of brucellosis vaccine. Finally, 19 brucellosis-resistant group (BRG) sheep and 22 brucellosis-susceptible group sheep (BSG) were screened for whole-genome sequencing. Using the fixation index, Fisher’s exact test, and chi-square test, a total of 205 candidate SNP sites were identified. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested that 138 candidate genes were significantly enriched in adherens junction ( CTNNA3 , PARD3 , and PTPRM ), cell adhesion molecules ( NLGN1 , CNTNAP2 , NCAM1 , and PTPRM ), salivary secretion ( LOC101102109 , PRKG1 , and ADCY2 ), and hippo signaling pathway ( CTNNA3 , YAP1 , and PARD3 ). These findings provide valuable molecular markers for brucellosis resistance breeding in sheep and novel insights into the genetic mechanism of brucellosis resistance.
Type of Medium:
Online Resource
ISSN:
1664-8021
DOI:
10.3389/fgene.2021.653927
DOI:
10.3389/fgene.2021.653927.s001
DOI:
10.3389/fgene.2021.653927.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2606823-0
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