In:
Evidence-Based Complementary and Alternative Medicine, Wiley, Vol. 2018, No. 1 ( 2018-01)
Abstract:
In this study, optimization of enzyme‐assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated. It was found that the optimum extraction conditions were 3% of enzyme concentration ( X 1 ), 30 of liquid‐to‐solid ratio ( X 2 ), 56°C of extraction temperature ( X 3 ), 200W of ultrasonic power ( X 4 ), 10 min of extraction time (X 5 ), and 5 of pH value ( X 6 ). Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%). The crude HCP was further purified using DEAE‐52 and Sephadex G‐150 gel column, and a major polysaccharide fraction from HCP, designed as HCP‐1a with molecular weight of 33.9 kDa, was obtained. The HCP and HCP‐1a were characterized by chemical analysis, FT‐IR, and HPLC. For antioxidant activities in vitro , HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe 2+ chelating activities. In subsequent immunostimulatory studies, significantly decreased NO, IL‐1 β , and TNF‐ α concentrations were observed in both of HCP and HCP‐1a treated RAW264.7 cells. Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
DOI:
10.1155/ecam.v2018.1
DOI:
10.1155/2018/8617070
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2148302-4
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