In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 3 ( 2022-03-29)
Abstract:
A Gram-stain-negative, non-motile, aerobic, yellow, convex, rod-shaped mesophilic bacterial strain, designated strain D33 T , was isolated from rhizosphere soil of ancient mulberry in Dezhou city, Shandong province, PR China. The strain grew at 8–37 °C (optimum, 30 °C), pH 4–9 (optimum, pH 7) and growth occurred at 0.5–5.5 % (w/v) NaCl (optimally at 1 %). The results of the phylogenetic analyses of 16S rRNA gene and whole genome sequences indicated that D33 T was closely related to members of the genus Flavobacterium and had the highest 16S rRNA gene sequence similarity with ‘ Flavobacterium agri ’ KACC 19300 (95.4 %), Flavobacterium ichthyis NST-5 T (94.6 %), Flavobacterium ahnfeltiae KCTC 32467 T (93.6 %) and Flavobacterium longum JCM 19141 T (93.6 %). The genome size of D33 T was 3.8 Mb and the DNA G+C content was 48.0 mol%. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH) and average amino acid identity (AAI) values among D33 T and reference strains were lower than the threshold values for species delineation. The only respiratory quinone of D33 T was menaquinone 6 (MK-6). The predominant fatty acids ( 〉 5 %) were C 15:0, C 16 : 0 , C 18 : 0 , iso-C 15:0, iso–C 17 : 0 3-OH, anteiso-C 15 : 0 and summed feature 9 . The polar lipid profile contained phosphatidylethanolamine, two unidentified aminophospholipids, three unidentified aminolipids and two unidentified lipids. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that D33 T is a representative of a novel species of the ge nus Flavobacterium , for which the name Flavobacterium selenitireducens sp. nov. is proposed. The type strain is D33 T (=GDMCC 1.1946 T =KACC 22131 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005304
Language:
English
Publisher:
Microbiology Society
Publication Date:
2022
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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