In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_5 ( 2015-05-01), p. 1542-1549
Abstract:
A novel Gram-stain-negative, rod-shaped, gliding, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated FA350 T , was isolated from coastal sediment from Xiaoshi Island, Weihai, China. Strain FA350 T showed growth on modified nutrient agar supplemented with 0.1 % d -(+)-trehalose and with distilled water replaced by seawater. Optimal growth occurred at 33 °C and pH 8.5 with 4 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain FA350 T belongs to a novel bacterial order in the class Deltaproteobacteria , and the most closely related type strains belong to the order Desulfuromonadales , with 85.1–85.6 % 16S rRNA gene sequence similarity. The polar lipid profile of the novel strain consisted of phosphatidylethanolamine, phosphatidylglycerol and two unknown phospholipids. Major cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 and iso-C 17 : 1 ω10 c and menaquinone MK-7 was the sole respiratory quinone. The DNA G+C content of strain FA350 T was 60.3 mol%. The isolate and closely related environmental clones formed a novel order-level clade in the class Deltaproteobacteria . Comparative analysis of 16S rRNA gene sequences and characterization indicated that strain FA350 T may represent a novel order of the Deltaproteobacteria . Here, we propose the name Bradymonas sediminis gen. nov., sp. nov. to accommodate strain FA350 T . The type strain of Bradymonas sediminis is FA350 T ( = DSM 28820 T = CICC 10904 T ); Bradymonadales ord. nov. and Bradymonadaceae fam. nov. are also proposed to accommodate the novel taxon.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.000135
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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