In:
Circulation Research, Ovid Technologies (Wolters Kluwer Health), Vol. 77, No. 5 ( 1995-11), p. 1024-1029
Abstract:
Abstract The effects of oral glucose challenge on plasma glucose concentration, plasma insulin concentration, arterial blood pressure, cytosolic pH (pH i ), cytosolic free Na + concentration ([Na + ] i ), and cellular Na + -H + exchange activity were investigated in 16 healthy subjects. The pH i , [Na + ] i , and Na + -H + exchange activity were measured in intact lymphocytes by using the fluorescent dye technique. The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na + -H + exchange activity, measured as change of pH i per second (control [0 hours], 5.20±0.53×10 −3 dpH i /s; 1 hour after glucose administration, 8.28±1.07×10 −3 dpH i /s; 2 hours after glucose administration, 8.15±1.18×10 −3 dpH i /s; P =.002). The lymphocytic Na + -H + exchange was significantly correlated with plasma glucose concentration ( r =.357, P =.041). During steady state euglycemic hyperinsulinemic clamp, the Na + -H + exchange activity was not significantly changed compared with baseline values. The study shows that changes of blood glucose levels can induce an acute increase in Na + -H + exchange activity. Systolic blood pressure and Na + -H + exchange activity were significantly ( P 〈 .001) but weakly correlated during an oral glucose challenge.
Type of Medium:
Online Resource
ISSN:
0009-7330
,
1524-4571
DOI:
10.1161/01.RES.77.5.1024
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
1995
detail.hit.zdb_id:
1467838-X
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