In:
PROTEOMICS, Wiley, Vol. 9, No. 3 ( 2009-02), p. 793-799
Abstract:
Comparative proteomics was applied to three vegetative organs of Brassica napus , the leaf, stem, and root using 2‐DE. Among the 〉 1600 analyzed spots, 43% were found to be common to all three organs, suggesting the existence of a “basal” or ubiquitous proteome composed of housekeeping proteins. The green organs, leaf, and stem, were closely related (∼80% common spots) while the root displayed more organ‐specific polypeptides (∼10%). Reference maps were established using MS, allowing the identification of 93, 385, and 266 proteins in leaf, stem, and root proteomes, respectively. Bioinformatic analyses were also performed; in silico functional categorization and cellular localization allow obtaining a precise picture of the cell molecular network within vegetative organs. These proteome maps can be explored using the PROTICdb software at the following address: http://bioinformatique.moulon.inra.fr/proticdb/web_view/.
Type of Medium:
Online Resource
ISSN:
1615-9853
,
1615-9861
DOI:
10.1002/pmic.200800479
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
2037674-1
SSG:
12
Bookmarklink