In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-9), p. e0254429-
Abstract:
Protein–metabolite interactions play an important role in the cell’s metabolism and many methods have been developed to screen them in vitro . However, few methods can be applied at a large scale and not alter biological state. Here we describe a proteometabolomic approach, using chromatography to generate cell fractions which are then analyzed with mass spectrometry for both protein and metabolite identification. Integrating the proteomic and metabolomic analyses makes it possible to identify protein-bound metabolites. Applying the concept to the thermophilic fungus Chaetomium thermophilum , we predict 461 likely protein-metabolite interactions, most of them novel. As a proof of principle, we experimentally validate a predicted interaction between the ribosome and isopentenyl adenine.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0254429
DOI:
10.1371/journal.pone.0254429.g001
DOI:
10.1371/journal.pone.0254429.g002
DOI:
10.1371/journal.pone.0254429.g003
DOI:
10.1371/journal.pone.0254429.g004
DOI:
10.1371/journal.pone.0254429.g005
DOI:
10.1371/journal.pone.0254429.s001
DOI:
10.1371/journal.pone.0254429.s002
DOI:
10.1371/journal.pone.0254429.s003
DOI:
10.1371/journal.pone.0254429.s004
DOI:
10.1371/journal.pone.0254429.s005
DOI:
10.1371/journal.pone.0254429.s006
DOI:
10.1371/journal.pone.0254429.s007
DOI:
10.1371/journal.pone.0254429.s008
DOI:
10.1371/journal.pone.0254429.s009
DOI:
10.1371/journal.pone.0254429.s010
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
Bookmarklink