In:
Frontiers in Cardiovascular Medicine, Frontiers Media SA, Vol. 10 ( 2023-5-5)
Abstract:
Atherosclerosis is the underlying cause of many cardiovascular diseases, such as myocardial infarction or stroke. B cells, and their production of pro- and anti-atherogenic antibodies, play an important role in atherosclerosis. In B cells, TRAF2 and NCK-interacting Kinase (TNIK), a germinal center kinase, was shown to bind to TNF-receptor associated factor 6 (TRAF6), and to be involved in JNK and NF-κB signaling in human B cells, a pathway associated with antibody production. Objective We here investigate the role of TNIK-deficient B cells in atherosclerosis. Results ApoE −/− TNIK fl/fl ( TNIK BWT ) and ApoE −/− TNIK fl/fl CD19-cre ( TNIK BKO ) mice received a high cholesterol diet for 10 weeks. Atherosclerotic plaque area did not differ between TNIK BKO and TNIK BWT mice, nor was there any difference in plaque necrotic core, macrophage, T cell, α-SMA and collagen content. B1 and B2 cell numbers did not change in TNIK BKO mice, and marginal zone, follicular or germinal center B cells were unaffected. Total IgM and IgG levels, as well as oxidation specific epitope (OSE) IgM and IgG levels, did not change in absence of B cell TNIK. In contrast, plasma IgA levels were decreased in TNIK BKO mice, whereas the number of IgA + B cells in intestinal Peyer's patches increased. No effects could be detected on T cell or myeloid cell numbers or subsets. Conclusion We here conclude that in hyperlipidemic ApoE −/− mice, B cell specific TNIK deficiency does not affect atherosclerosis.
Type of Medium:
Online Resource
ISSN:
2297-055X
DOI:
10.3389/fcvm.2023.1171764
DOI:
10.3389/fcvm.2023.1171764.s001
DOI:
10.3389/fcvm.2023.1171764.s002
DOI:
10.3389/fcvm.2023.1171764.s003
DOI:
10.3389/fcvm.2023.1171764.s004
DOI:
10.3389/fcvm.2023.1171764.s005
DOI:
10.3389/fcvm.2023.1171764.s006
DOI:
10.3389/fcvm.2023.1171764.s007
DOI:
10.3389/fcvm.2023.1171764.s008
DOI:
10.3389/fcvm.2023.1171764.s009
DOI:
10.3389/fcvm.2023.1171764.s010
DOI:
10.3389/fcvm.2023.1171764.s011
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2781496-8
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