Format:
Online-Ressource
ISSN:
1437-4331
Content:
Objectives: This proof of concept study was aimed to validate the hypothesis that the time of positivization of SARS-CoV-2 self-performed rapid diagnostic tests (RDTs) may reflect the actual viral load in the specimen. Methods: A SARS-CoV-2 positive sample with high viral load was diluted and concomitantly assayed with molecular assay (Xpert Xpress SARS-CoV-2) and RDT (COVID-VIRO ALL IN RDT). The (mean cycle threshold; Ct) values and RDT positivization times of these dilutions were plotted and interpolated by calculating the best fit. The parameters of this equation were then used for converting the positivization times into RDT-estimated SARS-CoV-2 Ct values in routine patient samples. Results: The best fit between measured and RDT-estimated Ct values could be achieved with a 2-degree polynomial curve. The RDT-estimated Ct values exhibited high correlation (r=0.996) and excellent Deming fit (y=1.01 × x − 0.18) with measured Ct values. In 30 consecutive patients with positive RDT test, the correlation between RDT positivization time and measured Ct value was r=0.522 (p=0.003). The correlation of RDT-estimated and measured Ct values slightly improved to 0.577 (Deming fit: y=0.44 × x + 11.08), displaying a negligible bias (1.0; 95% CI, −0.2 to 2.2; p=0.105). Concordance of RDT-estimated and measured Ct values at the 〈20 cut-off was 80%, with 0.84 sensitivity and 0.73 specificity. Conclusions: This proof of concept study demonstrates the potential feasibility of using RDTs for garnering information on viral load in patients with acute SARS-CoV-2 infection.
In:
volume:61
In:
number:2
In:
year:2023
In:
pages:316-322
In:
extent:07
In:
Clinical chemistry and laboratory medicine, Berlin [u.a.] : De Gruyter, 1998-, 61, Heft 2 (2023), 316-322 (gesamt 07), 1437-4331
Language:
English
DOI:
10.1515/cclm-2022-0873
URN:
urn:nbn:de:101:1-2022121413252109694975
URL:
https://doi.org/10.1515/cclm-2022-0873
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2022121413252109694975
URL:
https://d-nb.info/1275528252/34
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