Format:
Online-Ressource
ISSN:
1522-2675
Content:
Abstract: The retinal chromophore (11Z)‐3‐diazo‐4‐oxoretinal (1) with two photo‐labile moieties has been synthesized by semi‐hydrogenation of an 11‐yne precursor with activated Zn in aqueous media. Incorporation of 1 into opsin yielded diazoketo rhodopsin (DK‐Rh), which, upon bleaching, gave rise to intermediates batho‐Rh, lumi‐Rh, meta‐Rh, and meta‐II‐Rh corresponding to those of native Rh but at lower temperatures. Photoaffinity labeling of DK‐Rh and these bleaching intermediates showed that the ionone ring cross‐linked to Trp265 of helix F in DK‐Rh and batho intermediate, and to Ala169 of helix D in lumi, meta‐I, and meta‐II intermediates. These results demonstrate the occurrence of large conformational changes along the visual transduction path, which, in turn, is responsible for activation of the G‐protein.
In:
volume:83
In:
number:9
In:
year:2000
In:
pages:2617-2628
In:
extent:12
In:
Helvetica chimica acta, New York, NY : Wiley-VCH, 1918-, 83, Heft 9 (2000), 2617-2628 (gesamt 12), 1522-2675
Language:
English
DOI:
10.1002/1522-2675(20000906)83:9〈2617::AID-HLCA2617〉3.0.CO;2-9
URN:
urn:nbn:de:101:1-2023102805280564279139
URL:
https://doi.org/10.1002/1522-2675(20000906)83:9〈2617::AID-HLCA2617〉3.0.CO;2-9
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023102805280564279139
URL:
https://d-nb.info/1307642942/34
URL:
https://doi.org/10.1002/1522-2675(20000906)83:9〈2617::AID-HLCA2617〉3.0.CO;2-9
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