Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: We describe a rapid method whereby isolated chloroplasts from Euglena gracilis can be separated in a Ficoll gradient according to their surface pK's by isoelectric focusing in one hour or less. The linear Ficoll gradient is made isotonic over the whole separation distance by addition of sucrose. No released proteins of the isolated chloroplasts are detectable after isoelectric focusing. Modifications of the chloroplast envelope during the cell cycle of Euglena gracilis are evident by alterations of their isoelectric points.
In:
volume:3
In:
number:3
In:
year:2005
In:
pages:174-175
In:
extent:2
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 3, Heft 3 (2005), 174-175 (gesamt 2), 1522-2683
Language:
English
DOI:
10.1002/elps.1150030313
URN:
urn:nbn:de:101:1-2024040806455772249969
URL:
https://doi.org/10.1002/elps.1150030313
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024040806455772249969
URL:
https://d-nb.info/1325474843/34
URL:
https://doi.org/10.1002/elps.1150030313
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