Format:
1 Online-Ressource (iv, 94 pages)
ISBN:
9781510605015
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9781510605022
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9781510605008
Series Statement:
SPIE. Spotlight SL23
Content:
This Spotlight reviews non-invasive optical methods to determine the blood flow velocity in microcirculation, with an emphasis on the most recent developments that may be routinely applied in cellular biology laboratories. The methods covered in this book include both scanning and whole-field optical imaging techniques based on endogenous or exogenous (fluorescent) contrast agents for plasma and red-blood-cell visualization, such as laser Doppler velocimetry, laser speckle contrast imaging, diffuse correlation spectroscopy, particle image velocimetry, and two-photon microscopy. Several methods have been developed to quantify the blood flow velocity within microvessels by exploiting the cross-correlation concept applied to optical scanning microscopes: dual-foci cross-correlation, spatio-temporal image correlation spectroscopy, line-scanning techniques, and a recently developed single raster-scanned xy-image technique (FLICS). The basic operational principles, the advantages and disadvantages, and the technical issues of these methods will be discussed and compared, and their applications in biomedical research will be presented
Content:
1. Introduction -- 2. Laser speckle contrast imaging: 2.1. Principles; 2.2. Instrument configuration and data acquisition; 2.3. Applications -- 3. Doppler optical coherence tomography: 3.1. Principles; 3.2. Instrument configuration and data acquisition; 3.3. Applications -- 4. Diffuse correlation spectroscopy: 4.1. Principles; 4.2. Instrument configuration and data acquisition; 4.3. Applications -- 5. Multiplexed and image correlation methods: video-based acquisition: 5.1. Dual focus cross-correlation; 5.2. Particle image velocimetry; 5.3. Spatiotemporal image correlation spectroscopy; 5.4. Applications; 5.5. Other related methods -- 6. Space–time diagrams and image correlation methods: scanning-based acquisition and detection: 6.1. Line-scan and arbitrary path blood flow measurements; 6.2. Flow image correlation spectroscopy; 6.3. Instrument configuration and data acquisition; 6.4. Applications -- References
Note:
"SPIE Digital Library."--Website
,
Includes bibliographical references (pages 64-94)
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Restricted to subscribers or individual electronic text purchasers
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Mode of access: World Wide Web.
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System requirements: Adobe Acrobat Reader.
Language:
English
Keywords:
Electronic books
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