Format:
Online-Ressource
ISSN:
1460-2075
Content:
Transcripts for a new form of Sox5, called L‐Sox5, and Sox6 are coexpressed with Sox9 in all chondrogenic sites of mouse embryos. A coiled‐coil domain located in the N‐terminal part of L‐Sox5, and absent in Sox5, showed 〉90% identity with a similar domain in Sox6 and mediated homodimerization and heterodimerization with Sox6. Dimerization of L‐Sox5/Sox6 greatly increased efficiency of binding of the two Sox proteins to DNA containing adjacent HMG sites. L‐Sox5, Sox6 and Sox9 cooperatively activated expression of the chondrocyte differentiation marker Col2a1 in 10T1/2 and MC615 cells. A 48 bp chondrocyte‐specific enhancer in this gene, which contains several HMG‐like sites that are necessary for enhancer activity, bound the three Sox proteins and was cooperatively activated by the three Sox proteins in non‐chondrogenic cells. Our data suggest that L‐Sox5/Sox6 and Sox9, which belong to two different classes of Sox transcription factors, cooperate with each other in expression of Col2a1 and possibly other genes of the chondrocytic program.
In:
volume:17
In:
number:19
In:
year:1998
In:
pages:5718-5733
In:
extent:16
In:
European Molecular Biology Organization, The EMBO journal, Heidelberg : EMBO Press, 1982-, 17, Heft 19 (1998), 5718-5733 (gesamt 16), 1460-2075
Language:
English
DOI:
10.1093/emboj/17.19.5718
URN:
urn:nbn:de:101:1-2024010506593643357567
URL:
https://doi.org/10.1093/emboj/17.19.5718
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024010506593643357567
URL:
https://d-nb.info/1315110423/34
URL:
https://doi.org/10.1093/emboj/17.19.5718
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