Format:
Online-Ressource
ISSN:
1615-9861
Content:
Abstract: Among the integrative gene therapy vectors developed to date, human immunodeficiency virus type 1 (HIV‐1)‐derived lentiviral vectors (LV) are distinguished by their capacity to infect both dividing and non‐dividing cells. Recombinant LV particles contain viral proteins necessary for their packaging, infectious and integrating functions. Like the parental HIV‐1 virus they are able to acquire various cellular proteins, but the number and localisation of these proteins are poorly characterised. In the present study we used 2‐DE followed by MALDI‐TOF to quantify the protein content of several types of vesicular stomatitis virus G‐pseudotyped LV including those that were extensively purified in the perspective of clinical gene therapy studies. A proteinase K treatment was used to distinguish between cellular proteins incorporated into virions (I‐proteins) and those co‐purified with vectors (C‐proteins). We found 10 C‐proteins and 18 I‐proteins associated with LV. Copy numbers for these core I‐proteins varied from 5 (AIP‐1/ALIX) to 280 (Cyclophilin A) per vector particle. Three novel I‐proteins, guanine nucleotide‐binding protein 2, L‐lactate dehydrogenase B chain and hnRNP core protein A1, were found. This study defines for the first time, the protein stoichiometry of infectious HIV‐1‐derived LV particles.
In:
volume:9
In:
number:14
In:
year:2009
In:
pages:3666-3676
In:
extent:11
In:
Proteomics, Weinheim : Wiley VCH, [2001]-, 9, Heft 14 (2009), 3666-3676 (gesamt 11), 1615-9861
Language:
English
DOI:
10.1002/pmic.200800747
URN:
urn:nbn:de:101:1-2023042605161564823017
URL:
https://doi.org/10.1002/pmic.200800747
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023042605161564823017
URL:
https://d-nb.info/1287021190/34
URL:
https://doi.org/10.1002/pmic.200800747
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