Format:
Online-Ressource
ISSN:
1521-4141
Content:
Abstract: Dendritic cell‐specific intercellular adhesion molecule‐grabbing non‐integrin (DC‐SIGN), a specific C‐type lectin expressed on DC, binds and transmits different pathogens to susceptible cells. In the present study, we examined the role of DC‐SIGN in the capture of human papillomavirus (HPV) pseudovirions and activation of DC. We demonstrate that HPV virus‐like particles (VLP) bind to DC‐SIGN expressed on transfected Raji cells and that antibodies against DC‐SIGN block this interaction. DC take up VLP, which activate expression of costimulatory markers and cytokines/chemokines. Although our results indicate that DC‐SIGN is not the major receptor for VLP in DC, this interaction contributes to the activation of DC surface antigens (HLA class I) and of various cytokines/chemokines, particularly TNF‐α, IL‐6, and RANTES. Induction of these markers in DC by VLP was significantly abrogated when binding to DC‐SIGN was blocked by anti‐DC‐SIGN antibodies. These results suggest that DC‐SIGN has a functional role in DC activation induced by HPV‐16 L1‐VLP, and thus highlight new aspects of DC interactions with HPV VLP.
In:
volume:36
In:
number:2
In:
year:2006
In:
pages:437-445
In:
extent:9
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, 36, Heft 2 (2006), 437-445 (gesamt 9), 1521-4141
Language:
English
DOI:
10.1002/eji.200535068
URN:
urn:nbn:de:101:1-2023090906131132425400
URL:
https://doi.org/10.1002/eji.200535068
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023090906131132425400
URL:
https://d-nb.info/1301977039/34
URL:
https://doi.org/10.1002/eji.200535068
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