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  • 1
    UID:
    (DE-627)1727000366
    ISSN: 1533-2675
    In: Journal of relationship marketing, Philadelphia, PA : Routledge, Taylor & Francis Group, 2002, 19(2020), 3, Seite 165-181, 1533-2675
    In: volume:19
    In: year:2020
    In: number:3
    In: pages:165-181
    Language: English
    Keywords: Aufsatz in Zeitschrift
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  • 2
    Online Resource
    Online Resource
    Routledge
    UID:
    (DE-605)HT021784612
    Format: 1 Online-Ressource
    ISBN: 9781351142045
    Additional Edition: 9780815350965
    Language: Undetermined
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  • 3
    Online Resource
    Online Resource
    Saarbrücken : 金琅学术出版社
    UID:
    (DE-101)1078675589
    Format: Online-Ressource
    Edition: 1. Aufl.
    ISBN: 9783639819106 , 3639819101
    Note: Lizenzpflichtig. - Vom Verlag als Druckwerk on demand und/oder als E-Book angeboten
    Language: English
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  • 4
    Online Resource
    Online Resource
    [Erscheinungsort nicht ermittelbar] : eScholarship, University of California
    UID:
    (DE-627)180314758X
    Content: Polymerase Chain Reaction (PCR) is a widely used technology in molecular biology for DNA amplification. To generate multiple copies of a DNA molecule, a pair of primers (two synthesized DNA sequences with a total length of 15-30 bases) are annealed to the boundaries of the targeted DNA molecule. Then, the new replicated DNA fragment elongates from one primer to the other.Though primers always hybridize to their respective complements within DNA sequences, primer pairs for targeted DNA sequences can also anneal non-targeted DNA fragments containing common DNA sub-sequences also found in targeted DNA molecules. During the PCR process, primer pairs that offer high specificity and coverage rates for targeted fragments among all the copies are preferred.To provide primer pairs with high selectivity, several computational algorithms have been proposed. Most state-of-the-art algorithms take into account signature primers, or common short DNA fragments in the targeted DNA molecules. However, these algorithms do not account for the fact that during the PCR process in which primer pairs designed using signature primers are used, DNA fragments that do not have signature primers will not become amplified. These algorithms are, then, limited in various ways. Predicting primers' respective binding affinities is crucial in primer design because, during the PCR process, the annealing between the targeted DNA fragments and the primers with low binding affinity degenerates during the PCR process's thermal cycles. Because of this degeneration, targeted fragments expected to be reproduced by the primer pairs go missing during DNA amplification.It is important to note that a particular primer's nucleic acids do not contribute equally to the binding affinity. Specifically, this binding affinity is determined by the nucleic acids in the 3' end of the primer more than the nucleic acids in the 5' end. Existing algorithms typically oversimplify their predictions by either ignoring primers with high binding affinity or including primers with low binding affinity.To address current algorithms' limitations, we created PRISE2, a robust computational tool for sequence-selective PCR primer design. This innovative tool considers all subsequences of potential primer pairs to increase the coverage rate of the targeted fragments. This tool also provides a flexible mechanism with which to formulate positional bias when estimating primers' binding affinity. Importantly, the execution time of locating binding sites for all potential primers is positively proportion to the number of the subsequences. To accelerate searching for the binding sites, this tool clusters subsequences according to their sequence prefices to reduce the searching space. PRISE2 not only provides a user-friendly interface, but also offers full functionality for primer-design tasks. It was implemented using C++ and Qt frameworks to guarantee efficiency and achieve a cross-platform requirement.In applications where a collection of similar sequences need to be amplified using PCR, degenerate primers can be used to improve the efficiency and accuracy of amplification, since they can hybridize into multiple, unique DNA fragments. Conceptually, degenerate primers allow multiple bases at various positions. However, in reality, they are mixtures of regular primers that differ on certain bases. Specific degenerate primers' degeneracy refers to the number of regular primers in a mixture. Higher degeneracy allows a primer to amplify more targeted sequences simultaneously and also leads to low specificity for targeted sequences that adversely affect the quality and quantity of amplification. It is essential to find a good balance between high coverage and low degeneracy, a balance that a tool like PRISE2 helps achieve.For degenerate primer design, we proposed a new heuristic algorithm, RRD2P, to compute degenerate primer pairs with near-optimal coverage to targets under the specified degeneracy threshold. RRD2P runs in polynomial time and is confirmed to produce primer pairs with good coverage on three biological data sets. This production compares favorably with a similar tool called HYDEN. The fundamental goal driving RRD2P : to represent computing optimal primers as an integer linear program, solve their fractional relaxation, and then apply randomized rounding to obtain an integral solution.
    Note: Dissertation eScholarship, University of California 2015
    Language: English
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  • 5
    Online Resource
    Online Resource
    London : Routledge, Taylor & Francis
    UID:
    (DE-627)1765055245
    Format: 1 Online-Ressource (vi, 165 Seiten)
    ISBN: 9781003026174
    Series Statement: Routledge research on Taiwan 34
    Content: Introduction: What's in a name? : second-generation mainlander writing as a genre -- Constructing the mainlander : self, other, and homeland in Chu Tien-hsin's Everlasting and Yuan Chiung-chiung's This love, this life -- Seeking a new identity : Su Wei-chen's Leaving Tongfang and Chu Tien-hsin's In remembrance of my buddies from the military compound -- In quest of the absent mainlander father : family, history, and mainlander identity in Hao Yu-hsiang's The inn and Lo Yi-chin's The moon clan -- Inventing a Taiwanized Juancun : Lai Sheng-chuan and Wang Wei-chung's The village -- Happily ever after? : homecoming and mainlander identity in Chiang Hsiao-yun's Peach blossom well -- Conclusion and epilogue: Mainlander as an identity of in-betweenness.
    Additional Edition: 9780367458317
    Additional Edition: Erscheint auch als Druck-Ausgabe Huang, Phyllis Yu-ting, 1976 - Literary representations of "mainlanders" in Taiwan London : Routledge, 2021 9780367458317
    Language: English
    Keywords: Taiwan ; Migrantenliteratur
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  • 6
    UID:
    (DE-605)HT021817410
    Format: 1 Online-Ressource
    ISBN: 9781003026174
    Additional Edition: 9780367458317
    Language: Undetermined
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  • 7
    Book
    Book
    Abingdon, Oxon : Routledge, Taylor & Francis Group
    UID:
    (DE-603)519400194
    Format: 165 Seiten , 25 cm.
    ISBN: 0367648806 , 9780367648800
    Series Statement: Routledge research on Taiwan 34
    Content: "This book examines literary representations of mainlander identity articulated by Taiwan's second-generation mainlander writers, who share the common feature of emotional ambivalence between Taiwan and China. Closely analyzing literary narratives of Chinese civil war migrants and their descendants in Taiwan, a group referred to as "mainlanders" (waishengren), this book demonstrates that these Chinese migrants' ideas of "China" and "Chineseness" have adapted through time with their gradual settlement in the host land. Drawing upon theories of Sinophone Studies and memory studies, this book argues that during the three decades in which Taiwan moved away from the Kuomintang's authoritarian rule to a democratic society, mainlander identity was narrated as a transformation from a diasporic Chinese identity to a more fluid and elusive Sinophone identity. Characterized by the features of cultural hybridity and emotional in-betweenness, mainlander identity in the eight works explored contests the existing Sinocentric discourse of Chineseness. An important contribution to the current research on Taiwan's identity politics, this book will be of interest to academics in the field of Taiwan studies, Sinophone studies, Chinese migration, Taiwanese literature as well as Chinese literature in general
    Note: Index Seite 159-165.
    Language: English
    Keywords: Criticism, interpretation, etc
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  • 8
    UID:
    (DE-627)1736797301
    Format: vi, 165 Seiten
    ISBN: 9780367458317
    Series Statement: Routledge research on Taiwan series 34
    Content: Introduction: What's in a name? : second-generation mainlander writing as a genre -- Constructing the mainlander : self, other, and homeland in Chu Tien-hsin's Everlasting and Yuan Chiung-chiung's This love, this life -- Seeking a new identity : Su Wei-chen's Leaving Tongfang and Chu Tien-hsin's "In remembrance of my buddies from the military compound" -- In quest of the absent mainlander father : family, history, and mainlander identity in Hao Yu-hsiang's The inn and Lo Yi-chin's The moon clan -- Inventing a Taiwanized Juancun : Lai Sheng-chuan and Wang Wei-chung's The village -- Happily ever after? : homecoming and mainlander identity in Chiang Hsiao-yun's Peach blossom well -- Conclusion and epilogue: "Mainlander" as an identity of in-betweenness.
    Content: "This book examines literary representations of mainlander identity articulated by Taiwan's second-generation mainlander writers, who share the common feature of emotional ambivalence between Taiwan and China. Closely analyzing literary narratives of Chinese civil war migrants and their descendants in Taiwan, a group referred to as "mainlanders" (waishengren), this book demonstrates that these Chinese migrants' ideas of "China" and "Chineseness" have adapted through time with their gradual settlement in the host land. Drawing upon theories of Sinophone Studies and memory studies, this book argues that during the three decades in which Taiwan moved away from the Kuomintang's authoritarian rule to a democratic society, mainlander identity was narrated as a transformation from a diasporic Chinese identity to a more fluid and elusive Sinophone identity. Characterized by the features of cultural hybridity and emotional in-betweenness, mainlander identity in the eight works explored contests the existing Sinocentric discourse of Chineseness. An important contribution to the current research on Taiwan's identity politics, this book will be of interest to academics in the field of Taiwan studies, Sinophone studies, Chinese migration, Taiwanese literature as well as Chinese literature in general"--
    Note: Includes bibliographical references and index
    Additional Edition: 9781003026174
    Additional Edition: Erscheint auch als Online-Ausgabe Huang, Phyllis Yu-ting, - 1976- Literary representations of "mainlanders" in Taiwan London : Routledge, Taylor & Francis, 2021 9781003026174
    Language: English
    Keywords: Taiwan ; Migrantenliteratur
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  • 9
    Online Resource
    Online Resource
    Milton : Taylor & Francis Group
    UID:
    (DE-627)1738817776
    Format: 1 online resource (173 pages)
    ISBN: 9781000285475
    Series Statement: Routledge Research on Taiwan Ser.
    Content: Cover -- Half Title -- Series Page -- Title Page -- Copyright Page -- Table of Contents -- Acknowledgments -- Introduction: What's in a name? Second-generation mainlander writing as a genre 1 -- Mainlanders and mainlander literature in Taiwan -- Defining second-generation mainlander writers -- A genre of subjective identification -- From diaspora to the Sinophone -- Memory writing and Sinophone mainlander identity -- Chapter arrangement -- Notes -- References -- Chapter 1 Constructing the mainlander: Self, other, and homeland in Chu Tien-hsin's Everlasting ( 未 了 ) and Yuan Chiung-chiung's This Love, This Life ( 今 生 緣 ) -- Framing juancun literature -- Chu's and Yuan's novels as juancun literature -- Theorizing collective memory -- Unconscious representation of state ideology -- Under the gaze of the other -- Mandarin-speaking enclosures: Military dependents' villages -- Virtuous mainlander women versus indecent Taiwanese women -- China as a conceptual homeland -- Notes -- References -- Chapter 2 Seeking a new identity: Su Wei-chen's Leaving Tongfang ( 同 方 ) and Chu Tien-hsin's "In Remembrance of My Buddies from the Military Compound" ( 想 我 眷 村 的 兄 弟 們 ) -- Re-writing juancun in the context of the 1990s -- Juancun as a nostalgic home -- Juancun as sites of suffering -- Embracing a dismal past for the future -- Post-loyalist nostalgia -- Notes -- References -- Chapter 3 In the quest of the absent mainlander father: Family, history, and mainlander identity in Hao Yu-hsiang's The Inn 1 ( 旅 ) and Lo Yi-chin's The Moon Clan 2 ( 月 球 姓 氏 ) -- Frustrating father figures -- Individualizing the mainlander identity -- Father's story, my pedigree: Mainlander as a chosen identity -- In quest and inquest: An identity of ambivalence -- Personal narrative versus national narrative -- Notes -- References.
    Note: Description based on publisher supplied metadata and other sources
    Additional Edition: 9780367458317
    Additional Edition: Erscheint auch als Druck-Ausgabe 9780367458317
    Language: English
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  • 10
    UID:
    (DE-604)BV047355200
    Format: 165 Seiten
    ISBN: 9780367458317 , 9781003026174
    Series Statement: Routledge research on Taiwan series 34
    Content: Introduction: What's in a name? : second-generation mainlander writing as a genre -- Constructing the mainlander : self, other, and homeland in Chu Tien-hsin's Everlasting and Yuan Chiung-chiung's This love, this life -- Seeking a new identity : Su Wei-chen's Leaving Tongfang and Chu Tien-hsin's "In remembrance of my buddies from the military compound" -- In quest of the absent mainlander father : family, history, and mainlander identity in Hao Yu-hsiang's The inn and Lo Yi-chin's The moon clan -- Inventing a Taiwanized Juancun : Lai Sheng-chuan and Wang Wei-chung's The village -- Happily ever after? : homecoming and mainlander identity in Chiang Hsiao-yun's Peach blossom well -- Conclusion and epilogue: "Mainlander" as an identity of in-betweenness
    Content: "This book examines literary representations of mainlander identity articulated by Taiwan's second-generation mainlander writers, who share the common feature of emotional ambivalence between Taiwan and China. Closely analyzing literary narratives of Chinese civil war migrants and their descendants in Taiwan, a group referred to as "mainlanders" (waishengren), this book demonstrates that these Chinese migrants' ideas of "China" and "Chineseness" have adapted through time with their gradual settlement in the host land. Drawing upon theories of Sinophone Studies and memory studies, this book argues that during the three decades in which Taiwan moved away from the Kuomintang's authoritarian rule to a democratic society, mainlander identity was narrated as a transformation from a diasporic Chinese identity to a more fluid and elusive Sinophone identity. Characterized by the features of cultural hybridity and emotional in-betweenness, mainlander identity in the eight works explored contests the existing Sinocentric discourse of Chineseness. An important contribution to the current research on Taiwan's identity politics, this book will be of interest to academics in the field of Taiwan studies, Sinophone studies, Chinese migration, Taiwanese literature as well as Chinese literature in general
    Note: Includes bibliographical references and index , 2012
    Additional Edition: Erscheint auch als Online-Ausgabe ISBN 978-1-003-02617-4
    Additional Edition: ISBN 9781003026174
    Language: English
    Subjects: Comparative Studies. Non-European Languages/Literatures
    RVK:
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