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  • 1
    Book
    Book
    Totowa, NJ : Humana Press
    UID:
    (DE-604)BV023176495
    Format: XVII, 421 S.
    ISBN: 1588297799 , 9781588297792
    Series Statement: Methods in molecular biology 432
    Language: English
    Subjects: Biology
    RVK:
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    Keywords: Proteomanalyse ; Organell ; Aufsatzsammlung
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  • 2
    Book
    Book
    Totowa, NJ : Humana Press
    UID:
    (DE-627)550660356
    Format: XVII, 421 S. , Ill., graph. Darst. , 24cm
    ISBN: 9781588297792 , 1588297799
    Series Statement: Methods in molecular biology 432
    Note: Analysis of organelles by on-line two-dimensional liquid chromatography-tandem mass spectrometry / Edwin P. Romijn and John R. Yates III -- Purification and proteomic analysis of chloroplasts and their sub-organellar compartments / Daniel Salvi ... [et al.] -- Proteomic analysis of the stacked golgi complex / Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu -- Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow device / Hans Zischka ... [et al.] -- Preparation of respiratory chain complexes from Saccharomyces cerevisiae wild-type and mutant mitochondria : activity measurement and subunit composition analysis / Claire Lemaire and Geneviève Dujardin -- Mitochondrial proteomics : analysis of a whole mitochondrial extract with two-dimensional electrophoresis / Thierry Rabilloud -- Purification and proteomic analysis of the mouse liver mitochondrial inner membrane / Sandrine Da Cruz and Jean-Claude Martinou -- Subcellular fractionation and proteomics of nuclear envelopes / Laurence Florens, Nadia Korfali, and Eric C. Schirmer -- Purification and proteomic analysis of a nuclear-insoluble protein fraction / Tsuneyoshi Horigome, Kazuhiro Furukawa, and Kohei Ishii -- Preparation methods of human metaphase chromosomes for their proteome analysis / Kiichi Fukui, Hideaki Takata, and Susumu Uchiyama -- Purification and proteomic analysis of plant plasma membranes / Erik Alexandersson ... [et al.] -- Protocol to enrich and analyze plasma membrane proteins from frozen tissues / Jacek R. Wiśniewski -- Proteomic characterization of membrane protein topology / Adele R. Blackler and Christine C. Wu -- Free flow isoelectric focusing : a method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes / Markus Islinger and Gerhard Weber -- Use of gas-phase fractionation to increase protein identifications : application to the peroxisome / Jacob Kennedy and Eugene C. Yi -- Purification and proteomic analysis of lysosomal integral membrane proteins / Huiwen Zhang ... [et al.] -- Affinity purification of soluble lysosomal proteins for mass spectrometric identification / Sylvie Kieffer-Jaquinod ... [et al.] -- Purification and proteomic analysis of synaptic vesicles / Holly D. Cox and Charles M. Thompson -- Purification and proteomics analysis of pancreatic zymogen granule membranes / Xuequn Chen and Philip C. Andrews -- Isolation and proteomic analysis of chlamydomonas centrioles / Lani C. Keller and Wallace F. Marshall -- Purification and proteomic analysis of 20S proteasomes from human cells / Marie-Pierre Bousquet-Dubouch ... [et al.] -- Characterization of E. coli ribosomal particles : combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography-tandem mass spectrometry / Isabelle Iost ... [et al.] -- Assessment of organelle purity using antibodies and specific assays : the example of the chloroplast envelope / Daniel Salvi ... [et al.] -- Identifying bona fide components of an organelle by isotope-coded labeling of subcellular fractions : an example in peroxisomes / Marcello Marelli, Alexey I. Nesvizhskii, and John D. Aitchison -- Determination of genuine residents of plant endomembrane organelles using isotope tagging and multivariate statistics / Kathryn S. Lilley and Tom P.J. Dunkley -- Quantitative proteomic analysis to profile dynamic changes in the spatial distribution of cellular proteins / Wei Yan, Daehee Hwang, and Ruedi Aebersold -- Use of transcriptomic data to support organelle proteomic analysis / Wallace F. Marshall
    Additional Edition: 9781597450287
    Additional Edition: Erscheint auch als Online-Ausgabe Pflieger, Delphine Organelle Proteomics Totowa, NJ : Humana Press, 2008 9781597450287
    Language: English
    Subjects: Biology
    RVK:
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    Keywords: Organell ; Proteomanalyse ; Aufsatzsammlung
    URL: Cover
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  • 3
    Book
    Book
    London : Humana Press
    UID:
    (DE-603)194756831
    Format: XVII, 421 S. , graph. Darst.
    ISBN: 9781588297792 , 1588297799
    Series Statement: Methods in molecular biology 432
    Language: English
    Subjects: Biology
    RVK:
    RVK:
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    Keywords: Aufsatzsammlung
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  • 4
    Book
    Book
    Totowa, NJ :Humana Press,
    UID:
    (DE-602)almahu_BV023176495
    Format: XVII, 421 S.
    ISBN: 1-58829-779-9 , 978-1-58829-779-2
    Series Statement: Methods in molecular biology 432
    Language: English
    Subjects: Biology
    RVK:
    RVK:
    RVK:
    Keywords: Proteomanalyse ; Organell ; Aufsatzsammlung ; Aufsatzsammlung
    URL: Cover
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  • 5
    Book
    Book
    Totowa, NJ : Humana Press
    UID:
    (DE-605)HT015388568
    Format: XVII, 421 : Ill., graph. Darst.
    ISBN: 1588297799 , 9781588297792
    Series Statement: Methods in molecular biology 432
    Language: English
    Keywords: Organell ; Proteomanalyse ; Aufsatzsammlung
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  • 6
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    (DE-602)almahu_9949251526802882
    Format: 1 online resource (XVII, 421 p.)
    Edition: 1st ed. 2008.
    ISBN: 1-59745-028-6
    Series Statement: Methods in Molecular Biology, 432
    Content: Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.
    Note: Bibliographic Level Mode of Issuance: Monograph , Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry -- Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry -- Analysis of Organelles, Vesicles, and Protein Machineries -- Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments -- Proteomic Analysis of the Stacked Golgi Complex -- Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device -- Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria -- Mitochondrial Proteomics -- Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane -- Subcellular Fractionation and Proteomics of Nuclear Envelopes -- Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction -- Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis -- Purification and Proteomic Analysis of Plant Plasma Membranes -- Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues -- Proteomic Characterization of Membrane Protein Topology -- Free Flow Isoelectric Focusing -- Use of Gas-Phase Fractionation to Increase Protein Identifications -- Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins -- Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification -- Purification and Proteomic Analysis of Synaptic Vesicles -- Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes -- Isolation and Proteomic Analysis of Chlamydomonas Centrioles -- Purification and Proteomic Analysis of 20S Proteasomes from Human Cells -- Characterization of E. coli Ribosomal Particles -- Cellular, Genetic and Molecular Mechanisms of Plant Embryogenesis -- Assessment of Organelle Purity Using Antibodies and Specific Assays -- Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions -- Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics -- Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins -- Use of Transcriptomic Data to Support Organelle Proteomic Analysis. , English
    Additional Edition: ISBN 1-58829-779-9
    Language: English
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  • 7
    Online Resource
    Online Resource
    Totowa, NJ : Humana Press
    UID:
    (DE-603)321583450
    Format: XVII, 421 S.
    Edition: Online-Ausg. Berlin [u.a.] Springer 2013 Online-Ressource SpringerLink: Springer e-Books ISBN 9781597450287
    Edition: ISBN 1597450286
    Edition: Online-Ausg.
    ISBN: 9781588297792 , 9781597450287 (Sekundärausgabe) , 1597450286 (Sekundärausgabe)
    Series Statement: Methods in Molecular Biology™ 432
    Note: Online-Ausg.:
    Language: English
    Subjects: Biology
    RVK:
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    Keywords: Online-Publikation ; Aufsatzsammlung
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  • 8
    Online Resource
    Online Resource
    Totowa, NJ : Humana Press
    UID:
    (DE-627)1650789262
    Format: Online-Ressource (XVII, 421 p, digital)
    ISBN: 9781597450287
    Series Statement: Methods in Molecular Biology™ 432
    Content: Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry -- Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry -- Analysis of Organelles, Vesicles, and Protein Machineries -- Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments -- Proteomic Analysis of the Stacked Golgi Complex -- Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device -- Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria -- Mitochondrial Proteomics -- Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane -- Subcellular Fractionation and Proteomics of Nuclear Envelopes -- Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction -- Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis -- Purification and Proteomic Analysis of Plant Plasma Membranes -- Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues -- Proteomic Characterization of Membrane Protein Topology -- Free Flow Isoelectric Focusing -- Use of Gas-Phase Fractionation to Increase Protein Identifications -- Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins -- Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification -- Purification and Proteomic Analysis of Synaptic Vesicles -- Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes -- Isolation and Proteomic Analysis of Chlamydomonas Centrioles -- Purification and Proteomic Analysis of 20S Proteasomes from Human Cells -- Characterization of E. coli Ribosomal Particles -- Cellular, Genetic and Molecular Mechanisms of Plant Embryogenesis -- Assessment of Organelle Purity Using Antibodies and Specific Assays -- Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions -- Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics -- Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins -- Use of Transcriptomic Data to Support Organelle Proteomic Analysis.
    Content: Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.
    Additional Edition: 9781588297792
    Additional Edition: Buchausg. u.d.T. Organelle proteomics Totowa, NJ : Humana Press, 2008 9781588297792
    Additional Edition: 1588297799
    Language: English
    Subjects: Biology
    RVK:
    RVK:
    Keywords: Organell ; Proteomanalyse
    URL: Cover
    Library Location Call Number Volume/Issue/Year Availability
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  • 9
    Online Resource
    Online Resource
    Totowa, NJ : Humana Press
    UID:
    (DE-604)BV044952377
    Format: 1 Online-Ressource (XVII, 421 p)
    ISBN: 9781597450287
    Series Statement: Methods in Molecular Biology™ 432
    Content: Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study
    Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 9781588297792
    Language: English
    Subjects: Biology
    RVK:
    RVK:
    RVK:
    Keywords: Proteomanalyse ; Organell ; Aufsatzsammlung
    URL: Volltext  (URL des Erstveröffentlichers)
    Library Location Call Number Volume/Issue/Year Availability
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  • 10
    Online Resource
    Online Resource
    Totowa, NJ : Humana Press
    UID:
    (DE-627)666462461
    Format: Online-Ressource
    Edition: Online-Ausg. Electronic reproduction; Available via World Wide Web
    ISBN: 9781597450287
    Series Statement: Methods in Molecular Biology 432
    Note: Includes bibliographical references and index , Electronic reproduction; Available via World Wide Web
    Language: English
    Keywords: Organell ; Proteomanalyse
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