Format:
Online-Ressource
ISSN:
1521-4125
Content:
Abstract: Affinity separations rely on the highly specific binding between a protein in solution and an immobilized ligand to achieve a high degree of protein purification. A mathematical model including convection, diffusion, and rate kinetics is formulated to analyze the design and operation of affinity membrane bioseparation. The model equations are solved by orthogonal collocation method. Danckwerts' boundary conditions are used. The results obtained from model simulation show that the breakthrough of the protein is significantly influenced by Péclet number, feed protein concentration, Ligand number, Damköhler number, membrane thickness, and flow rate. Breakthrough profiles are quantitatively discussed in terms of protein recovery efficiency, ligand utilization efficiency, thickness of unused membrane, and width of the mass transfer zone.
In:
volume:19
In:
number:5
In:
year:2004
In:
pages:398-404
In:
extent:7
In:
Chemical engineering & technology, Weinheim : Wiley-VCH Verl.-Ges., 1987-, 19, Heft 5 (2004), 398-404 (gesamt 7), 1521-4125
Language:
English
DOI:
10.1002/ceat.270190503
URN:
urn:nbn:de:101:1-2023120103320471034963
URL:
https://doi.org/10.1002/ceat.270190503
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023120103320471034963
URL:
https://d-nb.info/1311809368/34
URL:
https://doi.org/10.1002/ceat.270190503
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