UID:
almafu_9961427390402883
Format:
1 streaming video file (46 min.) :
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digital, mono, SWF file, sound, color
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004616
Content:
Audio-visual presentation: Proteomics -- Quantitative mass spectrometry -- Stable isotope labelling -- Label free protein quantification -- PeptideAtlas project -- Targeted mass spectrometry via multiple reaction monitoring.
Note:
Retrieved April 17, 2024, from https://hstalks.com/bs/949/.
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Introduction -- Proteomics -- Rationale for accurate proteome quantification -- Quantitative proteomics - stable isotope labeling -- Gel free proteome analytical platform -- Protein identification strategy -- Accurate quantitation using isotope dilution -- Isotope coded affinity tags (ICAT) -- Quantitative proteomics by stable isotope dilution -- Stable isotope labeling strategies -- Quantitative proteomics technology -- Selective isolation from complex mixtures -- Quantitative proteomics via stable isotope tagging -- Label-free quantitative proteomics -- LC-MS label free proteomics method -- LC-MS data -- Reproducibility of LC-MS data -- SuperHirn and MasterMaps -- Feature map of four protein mixture -- Feature map of five protein mixture -- Feature map of protein no. 5 -- LC-MS results example 1 -- LC-MS results example 2 -- LC-MS results example 3 -- LC-MS approach to sample profiling -- Calibrated reference peptide probes -- Sequential proteomic experiments -- PeptideAtlas: different builds -- Targeted proteomics paradigm -- Multiple reaction monitoring (MRM) -- MRM quantification (internal standards) -- Duty cycle -- Scheduled MRMs -- Summary -- Conclusions.
Language:
English
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