Format:
1 Online-Ressource (vi, 105 Seiten, 18204 KB)
,
Illustrationen, Diagramme
Content:
Increasing concerns regarding the environmental impact of our chemical production have shifted attention towards possibilities for sustainable biotechnology. One-carbon (C1) compounds, including methane, methanol, formate and CO, are promising feedstocks for future bioindustry. CO2 is another interesting feedstock, as it can also be transformed using renewable energy to other C1 feedstocks for use. While formaldehyde is not suitable as a feedstock due to its high toxicity, it is a central intermediate in the process of C1 assimilation. This thesis explores formaldehyde metabolism and aims to engineer formaldehyde assimilation in the model organism Escherichia coli for the future C1-based bioindustry. The first chapter of the thesis aims to establish growth of E. coli on formaldehyde via the most efficient naturally occurring route, the ribulose monophosphate pathway. Linear variants of the pathway were constructed in multiple-gene knockouts strains, coupling E. coli growth to the activities of the key enzymes of the pathway. ...
Note:
kumulative Dissertation
,
Dissertation Universität Potsdam 2020
Additional Edition:
Erscheint auch als Druck-Ausgabe He, Hai Exploring and engineering formaldehyde assimilation Potsdam, 2020
Language:
English
Keywords:
Formaldehyd
;
Assimilation
;
Escherichia coli
;
Hochschulschrift
DOI:
10.25932/publishup-47386
URN:
urn:nbn:de:kobv:517-opus4-473867
URL:
https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-473867
URL:
https://d-nb.info/1219662658/34
Bookmarklink